| 缺氧预适应小鼠脑匀浆提取液对Hep G2细胞缺氧耐受性的影响 |
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| 引用本文： | 李宏,高文祥,卢国守,高钰琪. 缺氧预适应小鼠脑匀浆提取液对Hep G2细胞缺氧耐受性的影响[J]. 河南师范大学学报(自然科学版), 2007, 35(4): 127-131 |
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| 作者姓名： | 李宏 高文祥 卢国守 高钰琪 |
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| 作者单位： | 1. 第三军医大学,高原医学系高原生理学与病理生理学教研室,全军高原医学重点实验室,重庆,400038;重庆教育学院,生化系,重庆,400067
2. 第三军医大学,高原医学系高原生理学与病理生理学教研室,全军高原医学重点实验室,重庆,400038 |
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| 摘 要： | 目的:探讨缺氧预适应小鼠脑匀浆提取液(HP)对Hep G2细胞缺氧耐受性的影响.方法:用4种浓度的HP作用于缺氧(2%O2)培养HepG2细胞,以同浓度正常小鼠脑匀浆提取液(NP)作对照,检测了在缺氧培养后,Hep G2细胞的MTT值、早期凋亡率(Annexin V-FITC/PI双染流式细胞仪检测)、晚期凋亡率(Hoechst33258染色荧光显微镜检测).结果:低浓度HP对Hep G2细胞有保护作用,而高浓度HP对Hep G2细胞有损伤作用.结论:HP的作用有浓度依赖性和时间依赖性.
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| 关 键 词： | 缺氧预适应 小鼠 脑匀浆提取液 Hep G2细胞 缺氧耐受性 |
| 文章编号： | 1000-2367(2007)04-0127-04 |
| 修稿时间： | 2007-05-27 |
Effects of Brain Homogenate Extract of Hypoxia Preconditioned Mouse on the Tolerance of Hep G2 Cells to Hypoxia |
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| LI Hong,GAO Wen-xiang,LU Guo-shou,GAO Yu-qi. Effects of Brain Homogenate Extract of Hypoxia Preconditioned Mouse on the Tolerance of Hep G2 Cells to Hypoxia[J]. Journal of Henan Normal University(Natural Science), 2007, 35(4): 127-131 |
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| Authors: | LI Hong GAO Wen-xiang LU Guo-shou GAO Yu-qi |
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| Abstract: | Objective: To investigate the effect of brain homogenate extract of hypoxia preconditioned mouse(HP) on the tolerance of Hep G2 cells to hypoxia.Methods: HP at four concentrations were added into the cultures of HepG2 cells in hypoxia(2% O2),and brain homogenate extract at the same four concentrations of normal mouse(NP) were used as control.MTT assay conducted,and apoptotic percentages at early and late stages were detected respectively by means of Annexin V-FITC/PI double stained flow cytometry and Hoechst 33258 staining.Results: HP at low concentration showed protective effect on Hep G2 cells in hypoxia,whereas HP at high concentrations exerted detrimental effect on Hep G2 cells in hypoxia.Conclusion: The effects of HP depend on its concentrations and its treating time. |
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| Keywords: | hypoxia preconditioning mouse brain homogenate extract Hep G2 cells tolerance to hypoxia |
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