Spectroscopic investigation on the telomeric DNA base sequence repeat |
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Authors: | Email author" target="_blank">Sichuan?XuEmail author Xicheng?Ai Zhaoyong?Sun Qiyuan?Zhang Xingkang?Zhang |
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Institution: | (1) State Key Laboratory for Structural Chemistry of Unstable and Stable Species, Center of Molecular Science, Institute of Chemistry, Chinese Academy of Sciences, 100080 Beijing, China |
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Abstract: | Telomeres are protein-DNA complexes at the terminals of linear chromosomes, which protect chromosomal integrity and maintain
cellular replicative capacity. From single-cell organisms to advanced animals and plants, structures and functions of telomeres
are both very conservative. In cells of human and vertebral animals, telomeric DNA base sequences all are (TTAGGG)n. In the
present work, we have obtained absorption and fluorescence spectra measured from seven synthesized oligonucleotides to simulate
the telomeric DNA system and calculated their relative fluorescence quantum yields on which not only telomeric DNA characteristics
are predicted but also possibly the shortened telomeric sequences during cell division are implied. Oligonucleotide 5′-TTAGGGTTAGGG
holds a low relative fluorescence quantum yield and remarkable excitation energy innerconversion, which tallies with the telomeric
sequence of (TTAGGG)n. This result shows that telomeric DNA has a strong non-radiative or innerconvertible capability. |
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Keywords: | DNA telomere oligonucleotide fluorescence quantum yield |
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