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| 搅拌转速对Bacillus subtilis分批发酵碱性果胶裂解酶的影响 | |
| 引用本文： | 赵政,董云舟,堵国成,陈坚,高卫东. 搅拌转速对Bacillus subtilis分批发酵碱性果胶裂解酶的影响[J]. 东华大学学报(自然科学版), 2004, 30(5): 124-127 |
| 作者姓名： | 赵政 董云舟 堵国成 陈坚 高卫东 |
| 作者单位： | 1. 东华大学化学与化工学院,上海,200051 2. 江南大学生物工程学院工业生物技术教育部重点实验室,江苏,无锡,214036 3. 江南大学纺织服装学院,江苏,无锡,214063 |
| 基金项目： | 国家“863”项目(863-2001AA327090) |
| 摘 要： | 在5L小型发酵罐上，考察了不同搅拌转速对细胞生长和碱性果胶裂解酶生产的影响。根据细胞生物量、酶活水平、比细胞生长速率和比产物合成速率的变化情况，提出了两阶段搅拌转速控制策略：0～5h搅拌转速500r／min;5h后搅拌转速600r／min。PGL和PIVIGL酶活分别提高5．3％和15．1％；FGL和PMGL最大平均比生成速率分别提高15．9％和23．1％。 |
| 关 键 词： | 碱性果胶裂解酶 搅拌转速 Bacillus subtilis 聚半乳糖醛酸裂解酶 聚甲基半乳糖醛酸裂解酶 |
| 修稿时间： | 2003-06-09 |
Effect of Agitation Rate on Batch Alkaline Pectate Lyases Fermentation by Bacillus subtilis |
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| ZHAO Zheng,DONG Yun-zhou,DU Guo-cheng,CHEN Jian,GAO Wei-dong. Effect of Agitation Rate on Batch Alkaline Pectate Lyases Fermentation by Bacillus subtilis[J]. Journal of Donghua University, 2004, 30(5): 124-127 | |
| Authors: | ZHAO Zheng DONG Yun-zhou DU Guo-cheng CHEN Jian GAO Wei-dong |
| Affiliation: | ZHAO Zheng~1,DONG Yun-zhou~2,DU Guo-cheng~2,CHEN Jian~2,GAO Wei-dong~3 |
| Abstract: | Batch enzyme fermentation by Bacillus subtilis at different agitation rate ranged from 400 r/min to 600 r/min was studied in a 5 L fermentor. Based on time courses of the specific cell growth rate and specific alkaline pectate lyase production rate, a two-stage agitation rate control strategy was developed, in which agitation rate was controlled at 500 r/min at the first 5 h, then switched to 600 r/min afterwards. Applying this two-stage agitation rate control strategy, E_(1max) and E_(2max) could be improved by 5.3% and 15.1%, (?)_(1max) and (?)_(2max) could be improved by 15.9% and 23.1%, respectively. |
| Keywords: | alkaline pectate lyase agitation rate Bacillus subtilis PGL PMGL |
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