高压静电场对蒙古黄芪愈伤组织增殖和分化的影响

蒙古黄芪愈伤组织经场强为±1.0 kV/cm的高压静电场处理后生长速率明显提高,超氧化物酶(SOD)活性,过氧化氢酶(CAT) 活性和蛋白含量明显高于对照,过氧化物酶(POD)、吲哚乙酸(IAA)氧化酶活性和丙二醛(MDA)含量明显降低.正负高压静电场处理的结果存在着明显差异,正高压静电场能够有效地促进蒙古黄芪愈伤组织芽的分化,而负高压静电场能够有效地促进根的分化.

Effects of High Voltage Electrostatic Field on Growth and Differentiation of Astragalus membranaceus Bge. var.mongholicus(Bge.) Hisa Callus

Positive and negative high voltage electrostatic field(HVEF) with 1.0 kV/cm and-1.0 kV/cm intensity were used to treat Astragalus membranaceus Bge.var.mongholicus(Bge.) Hisa callus.The results showed that the callus growth rate obviously increased.The activities of SOD and CAT,content of protein were apparently higher than that of control respectively,whereas the activities of POD and IAA oxidase,content of MDA were respectively lower than those of control.There were significant differences between the positive and negative HVEF effects.The positive HVEF could promote the bud differentiation of A.membranaceus Bge.var.mongholicus(Bge.) Hisa callus,while the negative HVEF had a promotion role to the root differentiation.