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Density gradient centrifugation of cells separated from multicellular tumor spheroids
Authors:C. P. Sigdestad  D. J. Grdina  K. Ando
Affiliation:(1) Section of Experimental Radiotherapy, University of Texas System Cancer Center, M. D. Anderson Hospital & Tumor Institute, 6723 Bertner Avenue, 77030, Texas, Houston, USA;(2) Present address: Department of Therapeutic Radiology, University of Louisville, 500 S. Floyd St., 40202 Louisville, Kentucky, USA
Abstract:Summary Cells from a murine fibrosarcoma (FSa) have been grown in vitro as multicell tumor spheroids (MTS). The growth rate of these MTS was determined. Follwing selected periods of growth, MTS were made into a single cell suspension and separated on linear density gradients of Renografin. While only 1 population of cells was separated from small spheroids (400 mgrm diameter), at least 3 subpopulations of tumor cells were separated and isolated from large spheroids (800 mgrm in diameter).This investigation was supported in part by grants numbered CA-18628 and CA-06294, awarded by the National Cancer Institute, DHEW.
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