| 半夏凝集素基因的克隆 |
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| 引用本文： | 姚剑虹,孙小芬,唐克轩. 半夏凝集素基因的克隆[J]. 复旦学报(自然科学版), 2001, 40(4): 461-464 |
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| 作者姓名： | 姚剑虹 孙小芬 唐克轩 |
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| 作者单位： | 复旦大学生命科学学院 |
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| 基金项目： | Supported by ShanghaiScience and Technology Committee (Grant No. 98JC14035); Supported by China NationalTransgenic Plant Research and Commercialization Special Project (Grant No. J99-A-007);Supported by Sino-UK Science and Technology Collaboration Fund |
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| 摘 要： | 利用RACE－PCR技术从半夏花序中克隆出半夏凝集素的全长cDNA，通过比较半夏同其他天南星科植物的凝集素基因序列和推测的氨基酸序列，发现半夏凝集素基因编码一具有信号肽的前体蛋白，半夏凝集素同其他天南星科植物凝集素一样，为具有3个甘露糖专一结合盒的凝集素。
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| 关 键 词： | 天南星科 cDNA 凝集素 半夏 RACE－PCR 基因克隆 氨基酸序列 基因编码 |
| 文章编号： | 0427-7104 (2001) 04-0461-04 |
Molecular Cloning of Lectin Gene from Pinellia Ternata |
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| YAO Jian hong,SUN Xiao fen,TANG Ke xuan. Molecular Cloning of Lectin Gene from Pinellia Ternata[J]. Journal of Fudan University(Natural Science), 2001, 40(4): 461-464 |
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| Authors: | YAO Jian hong SUN Xiao fen TANG Ke xuan |
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| Abstract: | The full length cDNA of Pinellia ternata lectin gene was cloned from inflorescenceusing RACE PCR. Through comparative analysis of Pinellia ternata lectin gene and its deduced amino acid sequence with those of other Araceae species, Pinellia ternata lectin gene was found to encode a precursor lectin with signal peptide. Pinellia ternata lectin was a mannose binding lectin with three mannose binding boxes like lectins from other Araceae species. |
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| Keywords: | Araceae cDNA lectin Pinellia ternata RACE PCR |
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