| HPLC测定湖南省地产百合中薯蓣皂苷元含量 |
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| 引用本文： | 黄山林,童巧珍,周日宝,王朝晖,史艳霞,任旻琼,王珊,刘湘丹.HPLC测定湖南省地产百合中薯蓣皂苷元含量[J].湘南学院学报(自然科学版),2009(2):9-11. |
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| 作者姓名： | 黄山林 童巧珍 周日宝 王朝晖 史艳霞 任旻琼 王珊 刘湘丹 |
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| 作者单位： | [1]耒阳市新型农村合作医疗管理办公室,湖南耒阳421800 [2]湖南中医药大学,湖南长沙410208 |
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| 基金项目： | 国家科技支撑项目（2006BAI06A11～06）、湖南省中医院管理局资助项目（2008089） |
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| 摘 要： | 目的测定百合药材中薯蓣皂苷元的含量。方法Phenomenex C18色谱柱（4.6×250mm,5μm）;流速：0.7ml/min;柱温：30℃;检测波长：203nm;流动相：乙腈-水梯度洗脱。结果薯蓣皂苷元线性范围为0.04μg～1.60μg,r=0.9988,回收率为98.16%,RSD为2.07%。结论本方法灵敏、准确,可用于百合药材的质量控制。
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| 关 键 词： | HPLC 百合 薯蓣皂苷元 含量 |
Determine the content of diosgenin in Lily from Hunan province with HPLC |
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| Institution: | HUANG Shanlin, TONG Qiaozhen, ZHOU Ribao, et al (New Model Rural Area Co- operative Medical Care Manages Office, Hunan, Leiyang 421800, China) |
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| Abstract: | Objective To detennine the content of diosgenin in Lily. Methods Phenomenex C18 （4.6×250mm,5μm）colour spectntm post was used. Flow rate： 0.7 ml/min; Post temperature： 30 ℃ ; Check wavelength： 203 nm; Grad is washed coming off by mobile phase mixed of acetonitrile - water. Results The linear range of diosgenin was from 0.04 μg- 1.60 μg, r = 0.9988. The recovery of the added sample was 98.16%, RSD was 2.07%. Conclusion The method was simple, sensitive, accurate and be used as one of method for quality control of Lily. |
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| Keywords: | HPLC Lily Diosgenin Content |
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