| Protection of phosphatidylcholine to photosystem Ⅱ membrane during heat treatment |
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| 作者姓名： | LIDonghai XUQiang GONGYandao ZHANGXiufang ZHAONanming WANGKebin KUANGTingyun |
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| 作者单位： | [1]StateKeyLaboratoryofBiomembraneandMembraneBiotechnology,DepartmentofBiologicalSciencesandBiotecbnology,TsingbuaUniversity,Beijing100084,China [2]PhotosynthesisResearchCenter,InstituteofBotany,ChineseAcademyofSciences,Beijing100093,China |
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| 基金项目： | 国家重点基础研究发展计划(973计划)，国家自然科学基金 |
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| 摘 要： | Photosystem Ⅱ membrane was reconstituted with phosphatidylcholine (PC) with different kinds of fatty acyl chains and the protection of PC to photosystem Ⅱ (PS Ⅱ)membrane during heat treatment was investigated using oxygen electrode, variable fluorescence and circular dichroism (CD) spectroscopy. Heat treatment decreased the oxygen evolution rate and the F′v/Fm′ ratio of PS Ⅱ membrane and influenced CD spectra of PS Ⅱ membrane, but PC inhibited the effect of heat treatment on the oxygen evolution rate, the F′v/F′m ratio and CD spectra of PS Ⅱ membrane. The results indicate that PC can protect PS Ⅱ membrane against heat treatment and the alterations in the unsaturated fatty acid extent in PC can cause the changes of the protection ability.
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| 关 键 词： | 卵磷脂保护 光合体系Ⅱ薄膜 循环二色性 氧进化速率 热疗法 可变荧光性 |
| 收稿时间： | 2003-02-14 |
Protection of phosphatidylcholine to photosystem II membrane during heat treatment |
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| LIDonghai XUQiang GONGYandao ZHANGXiufang ZHAONanming WANGKebin KUANGTingyun.Protection of phosphatidylcholine to photosystem II membrane during heat treatment[J].Chinese Science Bulletin,2003,48(9):897-901. |
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| Authors: | Donghai Li Qiang Xu Yandao Gong Xiufang Zhang Nanming Zhao Kebin Wang Tingyun Kuang |
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| Institution: | LI Donghai, XU Qiang, GONG Yandao, ZHANG Xiufang, ZHAO Nanming, WANG Kebin & KUANG Tingyun State Key Laboratory of Biomembrane and Membrane Biotechnology, Department of Biological Sciences and Biotechnology, Tsinghua University, Beijing 100084, China Photosynthesis Research Center, Institute of Botany, Chinese Academy of Sciences, Beijing 100093, China |
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| Abstract: | Photosystem II membrane was reconstituted with phosphatidylcholine (PC) with different kinds of fatty acyl chains and the protection of PC to photosystem II (PS II) membrane during heat treatment was investigated using oxygen electrode, variable fluorescence and circular dichroism (CD) spectroscopy. Heat treatment decreased the oxygen evolution rate and the F′v/F m′ ratio of PS II membrane and influenced CD spectra of PS II membrane, but PC inhibited the effect of heat treatment on the oxygen evolution rate, the F′v/F′m ratio and CD spectra of PS II membrane. The results indicate that PC can protect PS II membrane against heat treatment and the alterations in the unsaturated fatty acid extent in PC can cause the changes of the protection ability. |
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| Keywords: | photosystem phosphatidylcholine (PC) oxygen evolution variable fluorescence circular dichroism (CD) phase transition heat treatment |
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