| AP－PCR在肿瘤DNA多态性研究中的应用 |
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| 引用本文： | 陈燃,杨树青,王世平,温俊娥,毛裕民.AP－PCR在肿瘤DNA多态性研究中的应用[J].复旦学报(自然科学版),1996(2). |
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| 作者姓名： | 陈燃 杨树青 王世平 温俊娥 毛裕民 |
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| 作者单位： | 复旦大学遗传学研究所 |
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| 摘 要： | 首次将ＡＰ－ＰＣＲ应用于ＤＮＡ多态性研究．研究ＡＰ－ＰＣＲ的反应程度，找到实现稳定扩增的适宜条件，并发现模板ＤＮＡ浓度的变化在相当范围内不会改变扩增图谱；对肿瘤、瘤周组织以及正常组织进行分析，在１１个引物中发现２个引物的扩增结果是多态的．初步结论：多态现象与癌变相关．
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| 关 键 词： | 肿瘤 组织 AP－PCR DNA多态性 癌变 |
Application of AP-PCR in polymorphic DNA analysis in carcinomas |
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| Chen Ran, Yang Shuqing, Wang Shiping, Wen June, Mao Yumin.Application of AP-PCR in polymorphic DNA analysis in carcinomas[J].Journal of Fudan University(Natural Science),1996(2). |
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| Authors: | Chen Ran Yang Shuqing Wang Shiping Wen June Mao Yumin |
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| Abstract: | P-PCR,a new molecular technique was used to amplify polymorphic DNA of carcinoma in this paper. We systematically studied the experimental protocols and found out an optimum program for carcinoma. Changes of the concentration of templet DNA does not change patterns of products of the AP-PCR within a wide range. 11primers were used,and 2 primers could amplify the polymorphic DNA of carcinoma. It was suggested that the polymorphic products might relevant to carcinogenesis. |
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| Keywords: | carcinoma AP-PCR polymorphic DNA tissue carcinogenesis |
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