| 包埋法固定SOD及固定化酶性质的研究 |
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| 引用本文： | 周诗毅,袁均林,贺红武,马玉涵,谭宏亮. 包埋法固定SOD及固定化酶性质的研究[J]. 华中师范大学学报(自然科学版), 2005, 39(1): 97-99 |
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| 作者姓名： | 周诗毅 袁均林 贺红武 马玉涵 谭宏亮 |
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| 作者单位： | 华中师范大学,生命科学学院,武汉,430079;华中师范大学,化学学院,武汉,430079 |
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| 基金项目： | 国家自然科学基金资助项目(200372023). |
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| 摘 要： | 采用包埋法固定SOD，并对固定化后的SOD的性质进行了研究，其结果表明，包埋剂聚丙烯酰胺的浓度为22．5％时，固定化的效果最好，固定化酶具有明显的热稳定，抗酸碱性，保存时间较长及可重复使用等优点．
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| 关 键 词： | SOD 固定化 包埋 |
| 文章编号： | 1000-1190(2005)01-0097-03 |
Immobilization of SOD with embedding and study on properties of the immobilized SOD |
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| ZHOU Shi-yi,YUAN Jun-lin,HE Hong-wu,MA Yu-han,TAN Hong-liang. Immobilization of SOD with embedding and study on properties of the immobilized SOD[J]. Journal of Central China Normal University(Natural Sciences), 2005, 39(1): 97-99 |
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| Authors: | ZHOU Shi-yi YUAN Jun-lin HE Hong-wu MA Yu-han TAN Hong-liang |
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| Affiliation: | ZHOU Shi-yi~1,YUAN Jun-lin~1,HE Hong-wu~2,MA Yu-han~1,TAN Hong-liang~1 |
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| Abstract: | In this study, embedding was applied to immobilize SOD and the properties of the immobilized SOD was studied. The result showed that as an embedding reagent, polyacrylamide exhibited the best immobilizing effect when its concentration was (22.5%.) As a consequence, the immobilized SOD had many distinct strongpoints such as being stable to heat, acid and alkali, the prolonged preserving duration and the capability of repeatitive use. |
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| Keywords: | SOD immobilization embedment |
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