| 分光光度法测定黔产绿茶中总黄酮的含量 |
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| 引用本文： | 傅文军,秦樊鑫,张丹.分光光度法测定黔产绿茶中总黄酮的含量[J].贵州师范大学学报(自然科学版),2005,23(4):105-107. |
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| 作者姓名： | 傅文军 秦樊鑫 张丹 |
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| 作者单位： | 1. 贵州师范大学,分析测试中心,贵州,贵阳
2. 贵州师范大学,理学院,贵州,贵阳,550001 |
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| 摘 要： | 利用黄酮类化合物与金属离子形成的鳌合物在510nm处有最大吸收度,采用有机溶剂乙醇索氏提取,建立绿茶叶中总黄酮含量的测定方法,测定波长510nm,芦丁（Rutin）为对照品,线性方程Y=0.01061X-0.0045,相关系数R=0.9999,加标平均回收率为97.58%,RSD=1.22%（n=11）.
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| 关 键 词： | 分光光度法 黔产绿茶 总黄酮 |
| 文章编号： | 1004-5570（2005）04-0105-03 |
| 收稿时间： | 2005-04-25 |
| 修稿时间： | 2005年4月25日 |
Determination of flavonoids in Guizhou green tea by spectrophotometry |
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| FU Wen-jun,QIN Fan-xin,ZHANG Dan.Determination of flavonoids in Guizhou green tea by spectrophotometry[J].Journal of Guizhou Normal University(Natural Sciences),2005,23(4):105-107. |
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| Authors: | FU Wen-jun QIN Fan-xin ZHANG Dan |
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| Institution: | 1. The Center of Analysis and Test; 2. School of Physics and Chemistry, Guizhou Normal University, Guiyang, Guizhou 550001 |
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| Abstract: | Compound of flavonoid compound and metal iron has the maximum absorption at wavelength of 510nm.Using ethanol extraction before determination method of flavonoids in green tea has been devised.Wavelength of determination is 510nm and standard material is rutin.Linear equation is Y= 0.010 61X-0.004 5.Linearity relation coefficient equals to 0.999 9 and recovery rate of added standard is 97.58%.RSD is 1.22%(n=11). |
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| Keywords: | spectrophotometry Guizhou green tea flavonoids |
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