| 蜜环菌胞外漆酶酶活力的分光光度法测定 |
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| 引用本文： | 吴涓,肖亚中,王怡平,李清彪. 蜜环菌胞外漆酶酶活力的分光光度法测定[J]. 厦门大学学报(自然科学版), 2001, 40(4): 893-898 |
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| 作者姓名： | 吴涓 肖亚中 王怡平 李清彪 |
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| 作者单位： | 1. 安徽大学生命科学学院,
2. 厦门大学化学工程系, |
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| 基金项目： | 安徽省科委自然科学基金资助项目(98212411) |
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| 摘 要： | 采用分光光度法测定密环菌（Armillaria mellea）胞外漆酶（Laccase）的活力，分别以2，2′－连氮－二（3－乙基苯并噻唑－6－磺酸）（简称ABTS）、丁香醛连氮、愈创木酚和邻联甲安为底物，比较了这4种底物对漆酸的敏感性，探讨了酶反应时间、温度、缓冲液及其pH值和底物与酶的用量等条件对漆酶催化反应的影响，实验结果表明，适宜的测酶条件可以确保漆酶活力的测定快速而准确。
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| 关 键 词： | 漆酶 底物 酶活力 蜜环菌 分光光度法 测定 |
| 文章编号： | 0438-0479(2001)04-0893-06 |
| 修稿时间： | 2000-06-02 |
Spectrophotometric Determination of Extracellular Laccase Activity of ArmillariaMellea |
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| WU Juan ,XIAO Ya zhong ,WANG Yi ping ,LI Qing biao. Spectrophotometric Determination of Extracellular Laccase Activity of ArmillariaMellea[J]. Journal of Xiamen University(Natural Science), 2001, 40(4): 893-898 |
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| Authors: | WU Juan XIAO Ya zhong WANG Yi ping LI Qing biao |
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| Affiliation: | WU Juan 1,XIAO Ya zhong 1,WANG Yi ping 1,LI Qing biao 2 |
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| Abstract: | Spectrophotometric determination was taken to determine extracellular laccase activity from Armiillaria mellea . ABTS, syringaldazine, guaiacol and tolidine was used as the substrate for laccase respectively. The effects of time, temperature, buffer and its pH, and the amount of substrate and enzyme on laccase catalytic reaction were studied. The experimental results showed that suitable determination could ensure rapid and accurate determination of laccase activity. |
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| Keywords: | laccase substrate enyzyme activity |
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