| 松树两种病原菌拮抗微生物的筛选 |
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| 引用本文： | 蒋萍,吴小芹,叶建仁,盛江梅.松树两种病原菌拮抗微生物的筛选[J].南京林业大学学报(自然科学版),2007,31(1):59-62. |
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| 作者姓名： | 蒋萍 吴小芹 叶建仁 盛江梅 |
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| 作者单位： | 南京林业大学森林资源与环境学院,江苏,南京,210037;新疆农业大学林学院,新疆,乌鲁木齐,830052;南京林业大学森林资源与环境学院,江苏,南京,210037 |
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| 基金项目： | 国家自然科学基金
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高等学校博士学科点专项科研项目 |
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| 摘 要： | 采用组织分离法从健康的和感染松枯梢病的松树针叶上分离了叶栖微生物1 862株.选取有代表性的菌株通过室内平皿对峙培养,初步筛选出对松枯梢病菌和松赤枯病菌具有较好生防潜力的菌株12株.其中,拮抗细菌菌株6株、酵母菌3株、丝状真菌木霉(Trichoderma sp.)3株,拮抗效果由强到弱依次为细菌、木霉、酵母菌,细菌无菌滤液的拮抗效果明显好于菌体本身.拮抗菌的筛选以相对抑菌效果为准,同时应考虑被抑制率和菌种本身具有的致病性.
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| 关 键 词： | 松枯梢病 松赤枯病 拮抗微生物 筛选 |
| 文章编号： | 1000-2006(2007)01-0059-04 |
| 修稿时间： | 2006-06-23 |
Screening of Antagonistic Microbes to Two Kinds of Pine Trees Pathogens |
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| JIANG Ping,WU Xiao-qin,YE Jian-ren,SHENG Jiang-mei.Screening of Antagonistic Microbes to Two Kinds of Pine Trees Pathogens[J].Journal of Nanjing Forestry University(Natural Sciences ),2007,31(1):59-62. |
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| Authors: | JIANG Ping WU Xiao-qin YE Jian-ren SHENG Jiang-mei |
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| Institution: | 1. College of Forest Resources and Evironment Nanjing Forestry University, Nanjing 210037,China; 2. Forestry College of Xinjiang Agricultural University, Urumqi 830052,China |
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| Abstract: | Through collecting,isolating and cultivating the pine phyllosphere microbes,several isolates were selected to test their antagonistic effect to Sphaeropsis sapinea and Pestalotiopsis sp.By the method of dual culture,the result showed that the isolates with obviously antagonistic ability to Sphaeropsis sapinea and Pestalotiopsis sp.contained six kinds of bacteria,three kinds of yeast and three kinds of Trichodermas sp.,which perhaps could be used for biological control.The antagonistic effect of bacteria is the highest,secondly Trichoderma sp.and yeast lastly.The standard for screening antagonistic fungus is relatively restraining the effect of a fungus.At the same time the restrained proportion and the characteristics leading to disease for the fungus should be considered. |
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| Keywords: | Sphaeropsis sapinea Pestalotiopsis sp Antagonistic microbe Screening |
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