Identification of factor XIII-A as a marker of alternative macrophage activation |
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Authors: | D Törőcsik H Bárdos L Nagy R Ádány |
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Institution: | (1) Department of Biochemistry and Molecular Biology, Faculty of Medicine, Medical and Health Science Centre, University of Debrecen, 4012 Debrecen, Hungary;(2) Department of Preventive Medicine, School of Public Health, Medical and Health Science Centre, University of Debrecen, Kassai str. 26, 4028 Debrecen, Hungary |
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Abstract: | Factor XIII subunit A of blood coagulation (FXIII-A) is known to be synthesized but not secreted by the monocyte/macrophage cell line. On the basis of its intracellular localization and substrate profile, FXIII-A is thought to be involved in certain intracellular processes. Our present study was designed to monitor the changes in FXIII-A gene expression and protein production in long-term culture of human monocytes during their differentiation into macrophages in the presence of activating agents (interleukin-4, interferon-γ, Mycobacterium bovis BCG) inducing classical and alternative activation pathways. By using quantitative RT-PCR and fluorescent image analysis at the single-cell level we demonstrated that the expression of FXIII-A both at the mRNA as well as at the protein level is inversely regulated during the two activation programmes. Here we conclude that FXIII-A expression is an intracellular marker for alternatively activated macrophages, while its absence in monocyte-derived macrophages indicates their classically activated state.Received 2 June 2005; received after revision 12 July 2005; accepted 22 July 2005 |
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Keywords: | Factor XIII-A macrophage gene expression alternative activation classical activation |
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