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| 新城疫病毒TL1株P、NP、L蛋白基因表达载体的构建及鉴定 | |
| 引用本文： | 王学理,李晓艳,关洪玉,任林柱,刘锴,殷小宇,闫广谋,王兴龙. 新城疫病毒TL1株P、NP、L蛋白基因表达载体的构建及鉴定[J]. 内蒙古民族大学学报(自然科学版), 2007, 22(4): 417-421 |
| 作者姓名： | 王学理 李晓艳 关洪玉 任林柱 刘锴 殷小宇 闫广谋 王兴龙 |
| 作者单位： | 1. 解放军军事医学科学院军事兽医研究所,吉林,长春,130062;内蒙古民族大学动物科技学院,内蒙古,通辽,028042;吉林大学畜牧兽医学院,吉林,长春,130062 2. 解放军军事医学科学院军事兽医研究所,吉林,长春,130062 3. 通辽市科尔沁区动物防疫监督检验所,内蒙古,通辽,028000 4. 解放军军事医学科学院军事兽医研究所,吉林,长春,130062;吉林大学畜牧兽医学院,吉林,长春,130062 |
| 基金项目： | 军事医学科学院基金;吉林省科技厅科技发展计划 |
| 摘 要： | 将新城疫病毒TL1株的P、NP、L基因通过RT-PCR方法从尿囊液中扩增后分别克隆进pGEM-Teasy载体,再分别亚克隆到真核表达载体pCI-neo上,命名为pCI-P、pCI-NP、pCI-L,通过酶切、PCR和测序验证克隆正确.纯化后的重组质粒单独转染BHK-21细胞,经间接免疫荧光试验检测到了P、NP、L蛋白的表达.新城疫病毒TL1株的P、NP、L基因的克隆成功,为即将进行的新城疫病毒的反向遗传操作奠定了基础. |
| 关 键 词： | 新城疫病毒 P、NP、L基因 间接免疫荧光 表达 |
| 文章编号： | 1671-0185（2007）04-0417-05 |
| 收稿时间： | 2007-07-11 |
| 修稿时间： | 2007-07-11 |
Construction and Identification of P,NP and L Genes Expression Vectors of Newcastle Disease Virus TL1 Strain |
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| WANG Xue-li,LI Xiao-yan,GUAN Hong-yu,REN Lin-zhu,LIU Kai,DUAN Xiao-yu,YAN Guang-mou,WANG Xing-long. Construction and Identification of P,NP and L Genes Expression Vectors of Newcastle Disease Virus TL1 Strain[J]. Journal of Inner Mongolia University for the Nationalities(Natural Sciences), 2007, 22(4): 417-421 | |
| Authors: | WANG Xue-li LI Xiao-yan GUAN Hong-yu REN Lin-zhu LIU Kai DUAN Xiao-yu YAN Guang-mou WANG Xing-long |
| Affiliation: | 1.The Military Veterinary Institute,Academy of Military Medical Sciences,Changchun 130062,China;2.Animal Science and Technology College,Inner Mongolia University For Nationalities,Tongliao 028000,China;3.College of Animal Science and Veterinary Medicine,Jilin University,Changchun 130062,China;4.Kerchin County Animal Supervision Institue of Tongliao City,Tongliao 028000,China |
| Abstract: | P,NP and L genes of Newcastle disease virus TL1 strain were amplified and cloned into pGEM-T easy vector and then subcloned into pCI-neo expression vector respectively.The positive recombinant plasmids were named pCI-P,pCI-NP and pCI-L after identification by restriction digestion,PCR amplification and sequencing analyses.Then the recombinant plasmids were purified and transfected into BHK-21 cells.Expression of P,NP and L genes were detected and confirmed by the indirect immunofluorescence analysis.This research may be helpful for further study of reverse genetics and functional genome of NDV. |
| Keywords: | Newcastle disease virus P,NP and L genes The indirect immunofluorescence Expression |
| 本文献已被 CNKI 维普 万方数据 等数据库收录！ | |