| 海洋石油烃降解菌群构建及其在降解过程中的动态分析 |
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| 引用本文： | 谭田丰 邵宗泽. 海洋石油烃降解菌群构建及其在降解过程中的动态分析[J]. 厦门大学学报(自然科学版), 2006, 45(5): 262-266 |
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| 作者姓名： | 谭田丰 邵宗泽 |
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| 作者单位： | [1]厦门大学生命科学学院,福建厦门361005 [2]国家海洋局第三海洋研究所海洋生物遗传资源重点实验室,福建厦门361005 |
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| 基金项目： | 中国大洋协会资源加工利用项目（DY105-04-02-06）,厦门市海洋与渔业局科技计划项目资助 |
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| 摘 要： | 有针对性地选择了实验室从油污染的样品中富集筛选出来的6株细菌构建出一个石油烃降解菌群，采用PCR-DGGE结合平板计数监测法研究了该菌群在石油烃降解过程中的菌群结构变化．结果表明，能产生生物乳化剂的不动杆菌PN3-2在混合菌群中是稳定优势菌，专一性利用烷烃的食烷菌B-5是菌群拥有持久高效降解能力不可缺少的菌株，而降解后期的优势菌铜绿假单胞菌可能对烷烃代谢产物的清除有重要作用．本实验为人工构建有机污染物降解菌群提供了实践经验，并为大规模的生物修复实践奠定了基础．
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| 关 键 词： | 石油污染 生物降解 菌群 PCR-DGGE |
| 收稿时间： | 2005-12-25 |
Construction of Marine Oil Biodegradation Bacterial Consortium and Its Dynamic Change in the Process of Oil Consumption |
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| TAN Tian-feng, SHAO Zong-ze. Construction of Marine Oil Biodegradation Bacterial Consortium and Its Dynamic Change in the Process of Oil Consumption[J]. Journal of Xiamen University(Natural Science), 2006, 45(5): 262-266 |
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| Authors: | TAN Tian-feng SHAO Zong-ze |
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| Affiliation: | 1. School of Life Sciences, Xiamen University, Xiamen 361005, China; 2. Key Laboratory of Marine Biogenetic Resources, The Third Institute of Oceanography, State Oceanic Administration, Xiamen 361005, China |
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| Abstract: | Six strains screened from oil contaminated area have been chosen to construct an oil biodegradation bacterial consortium, and the dynamic change of the consortium in the process of oil consumption has been monitored by PCR-DGGE and CFU method. The results indicated that the bioemulsan producing strain PN3-2 was a stable predominant strain in the consortium, the strain B-5 which usesd n-alkyl as the sole carbon source during the growth played an important role in the consortium to extend the degradation period, while 2HE-4 was a strain which might play an important role in metabolic intermediate of n-alkyl remove. This consortium can be used in a large-scale outdoor bioremediation test. |
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| Keywords: | petroleum pollution biodcgradation bacterial consortium PCR-DGGE GC-MS |
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