酶联免疫法快速检测牛奶及其制品中的黄曲霉毒素B1

为了建立乳及乳制品中黄曲霉毒素B1的快速检测方法,对酶联免疫吸附法(ELISA)快速检测牛奶及其制品中的黄曲霉毒素B1进行灵敏度、准确度、精密度、重复性、重现性等方法学评价试验,并用高效液相色谱法进行验证。结果ELISA的灵敏度为0.03ng/ml,加标回收率为95.0%～104.2%,精密度相对平均偏差与相对标准偏差均为1.0%～1.6%,重复性的相对标准偏差为3.15%,再现性的相对标准偏差为8.21%。样品测定结果ELISA与液相色谱法的相对标准偏差小于10%。ELISA可以较快速地、准确地、大批量地检测牛奶及其制品中的黄曲霉毒素B1。

Application of ELISA for Rapid Detection Aflatoxin B1 in Milk and Dairy Products

A enzyme-linked immunosorbent assay（ELISA） was established to rapidly detect aflatoxin B1 in milk and dairy products.The sensitivity,accuracy,precision,repetitiveness and reproduction of were aflatoxin B1 detection in milk and dairy products by ELISA methodological.High performance liquid chromatography was used to verify the aflatoxin B1 by evaluated ELISA.The sensitivity was 0.03ng/ml.The recovery of standard addition was 95.0% to 104.2%.The relative average deviation and relative standard deviation of precision（RSD） was both in the range of 1.0% to 1.6%.The RSD of repetitiveness and reproduction was 3.15% and 8.21% respectively.The RSD on validation of method between HPLC and ELISA was less than 10%.The residue of aflatoxin B1 in milk and dairy products can be rapidly and accurately detected on a mass scale with ELISA method.