| 响应曲面法优化莲子蛋白质的提取条件 |
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| 引用本文： | 刘永乐,王发祥,俞健,王建辉,李向红,武玮芸.响应曲面法优化莲子蛋白质的提取条件[J].长沙理工大学学报(自然科学版),2012,9(4):1-4,31. |
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| 作者姓名： | 刘永乐 王发祥 俞健 王建辉 李向红 武玮芸 |
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| 作者单位： | 长沙理工大学化学与生物工程学院,湖南长沙,410004 |
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| 基金项目： | 国家“十二五”科技支撑计划资助项目，国家自然科学基金资助项目 |
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| 摘 要： | 通过PB设计一最陡爬坡实验一响应曲面法，优化了莲子蛋白质的提取工艺，获得了其最佳工艺条件，即提取液SDS浓度为3．27％、DTT浓度为0．2％、pH＝12．0、液固比（V／W）为12：1、提取温度为30℃、提取时间90min、超声波辅助时间为21．5min．在此条件下，莲子蛋白质提取率可达92．27％．
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| 关 键 词： | 莲子 蛋白质 响应曲面法 提取 优化 |
Optimization of extraction technique of protein from lotus seed using response surface methodology |
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| LIU Yong-le,WANG Fa-xiang,YU Jian,WANG Jian-hui, LI Xiang-hong,WU Wei-yun.Optimization of extraction technique of protein from lotus seed using response surface methodology[J].Journal of Changsha University of Science and Technology:Natural Science,2012,9(4):1-4,31. |
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| Authors: | LIU Yong-le WANG Fa-xiang YU Jian WANG Jian-hui LI Xiang-hong WU Wei-yun |
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| Institution: | (School of Chemistry and Biological Engineering,Changsha University of Science and Technology,Changsha 410004,China) |
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| Abstract: | The extraction technique of proteins from lotus seed was optimized using Plack- ett-Burman design, path of steepest ascent and response surface methodology. The results showed that the optimum extraction condition was 3.27% SDS concentration, 0.2% DTT concentration, pH= 12.0, 12 ： I（V/W） of solvent/material ratio,and ultrasonic assistant treatment 21.5 min, extracting in 30℃ for 90 min. The extraction yield of protein from lo- tus seed could reach 92.27 % under this optimum extraction condition. |
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| Keywords: | lotus seed protein response surface methodology extraction optimization |
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