A small metalloribozyme with a two-step mechanism. |
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Authors: | T Pan O C Uhlenbeck |
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Institution: | Department of Chemistry and Biochemistry, University of Colorado, Boulder 80309. |
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Abstract: | An RNA molecule consisting of an asymmetric internal loop of six nucleotides can be rapidly and specifically cleaved by Pb2+ in the presence of Mg2+. The 5' cleavage product terminates with a 3' phosphomonoester generated from a 2',3'-cyclic phosphodiester reaction intermediate. This two-step reaction mechanism resembles that of many protein ribonucleases but has not previously been observed for reactions catalysed by RNA. |
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