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促性腺激素(FSH)对甾类激素合成调节作用的研究
引用本文:郭淑华. 促性腺激素(FSH)对甾类激素合成调节作用的研究[J]. 潍坊学院学报, 2005, 5(6): 72-74
作者姓名:郭淑华
作者单位:潍坊学院,山东,潍坊,261061
摘    要:首先分离出23日龄SD品系大鼠的卵巢颗粒细胞,然后进行无血清细胞培养,采用放射免疫的方法分别鉴定了不同剂量、不同培养时间促性腺激素(FSH)对颗粒细胞中甾类激素合成的影响,结果表明,甾类激素合成受FSH的影响,并且具有浓度和时间依赖性.

关 键 词:细胞培养  放射免疫  甾类激素
文章编号:1671-4288(2005)06-0072-03
收稿时间:2005-08-13
修稿时间:2005-08-13

The effect of FSH on steroids synthesis
GUO Shu-hua. The effect of FSH on steroids synthesis[J]. Journal of Weifang University, 2005, 5(6): 72-74
Authors:GUO Shu-hua
Abstract:Granulosa cells (GCs) were harvested from ovaries of DES-primed immature female rats and cultured, The cells were treated with various concentration of FSH for 24 h or 50 ng/ml FSH for different time spans as indicated; Progesterone and estradiol levels in the media were detected by RIA.. The results showed that Dose-dependent and Time-dependent increase of the steroids level by FSH in cultured rat primary GCs
Keywords:FSH
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