| 大球盖菇原生质体制备及紫外诱变 |
| |
| 引用本文： | 王谦,刘敏,张亚从.大球盖菇原生质体制备及紫外诱变[J].河北大学学报(自然科学版),2011,31(4):413-417. |
| |
| 作者姓名： | 王谦 刘敏 张亚从 |
| |
| 作者单位： | 河北大学生命科学学院,河北保定,071002 |
| |
| 基金项目： | 河北省科技厅支撑项目之子课题 |
| |
| 摘 要： | 对大球盖菇（Stropharia rugoso-annulata）原生质体制备的条件进行了研究,并确定了最佳条件组合:选用15 g/L的纤维素酶+15 g/L的蜗牛酶的混合酶来酶解菌丝,0.6 mol/L的甘露醇作为渗透压稳定剂,菌龄为3d,酶解温度30℃,酶解时间2.5h,在此条件下制得的原生质体产量最高达1.70×...
|
| 关 键 词： | 大球盖菇 原生质体 制备条件 紫外诱变 |
Protoplast Preparation and Ultraviolet Radiation Investigatio of Stropharia rugoso-annulata |
| |
| WANG Qian,LIU Min,ZHANG Ya-cong.Protoplast Preparation and Ultraviolet Radiation Investigatio of Stropharia rugoso-annulata[J].Journal of Hebei University (Natural Science Edition),2011,31(4):413-417. |
| |
| Authors: | WANG Qian LIU Min ZHANG Ya-cong |
| |
| Institution: | (College of Life Sciences,Hebei University,Baoding 071002,China) |
| |
| Abstract: | Conditions of preparating protoplast of Stropharia rugoso-annulata were studied.The result shows that the highest production rate of protoplast(1.70×107 mL-1) can be obtained on these conditions:3-day-old mecia,15 g/L cellulase and 15 g/L snailase admixture,dissolution in 0.6 mol/L mannitol,resolved at 30 ℃ for 2.5 h.Ultraviolet radiation inducing S.rugoso-annulata protplast was researched too.The conclusion is that 35 s is the irradiation time,deadly ratio is 73.33%. |
| |
| Keywords: | Stropharia rugoso-annulata protoplast preparation condition ultravilot radiation entice |
| 本文献已被 CNKI 万方数据 等数据库收录！ |
|
