| hSOD基因导入细胞抗超氧阴离子毒性的研究 |
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| 引用本文： | 吴晓英,KOMADA Fusao.hSOD基因导入细胞抗超氧阴离子毒性的研究[J].华南理工大学学报(自然科学版),2000,28(9):110-113. |
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| 作者姓名： | 吴晓英 KOMADA Fusao |
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| 作者单位： | [1]华南理工大学食品与生物工程学院,广东广州 [2]日本神户大学医学部,日本神户 |
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| 摘 要： | 研究了已获得稳定表达的ｈＳＯＤ基因导入细胞（ＣＭＶ－ＳＯＤ细胞）对百草枯（ｐａｒａｑｕａｔ）和黄嘌呤（Ｘ）／黄嘌呤氧化酶（ＸＯ）产生的超氧阴离子造成的细胞毒性的抵抗作用。结果表明：在百草枯和过氧化氢酶共存时，ＣＭＶ－ＳＯＤ细胞的存活率明显高于父本细胞，同时父本细胞过氧化脂质的产生数值显著高于ＣＭＶ－ＳＯＤ细胞；经过Ｘ／ＸＯ处理，ＣＭＶ－ＳＯＤ细胞的存活率为５３％，而父本细胞的存活率仅为４０％，同时
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| 关 键 词： | hSOD基因 超氧阴离子 细胞毒性 基因治疗 抵抗力 |
| 修稿时间： | 1999-09-28 |
A Study on Resistance of Transfected Cells with hSOD Gene to Superoxide Anion-Induced Cytotoxicity |
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| KOMADA Fusao.A Study on Resistance of Transfected Cells with hSOD Gene to Superoxide Anion-Induced Cytotoxicity[J].Journal of South China University of Technology(Natural Science Edition),2000,28(9):110-113. |
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| Authors: | KOMADA Fusao |
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| Abstract: | A study on the resistance of transfected cells with hSOD gene to superoxide a_nion_induced cytotoxicity caused by paraquat and xanthine (X)/xanthine oxidase (XO) is carried out in this paper. Results show that when exposed to paraquat and catalase, significantly more the CMV-SOD cells (hSOD gene transfection) survive than host cells. The production of lipid peroxidation in host cells increases more significantly after exposure to both paraquat and catalase than that in CMV-SOD cells. When exposed to X/XO, 53% CMV-SOD cells survive whereas 40% of host cells. The production of lipid peroxidation in host cells increases more significantly than in CMV-SOD cells. These results indicate that CMV-SOD cells is effective against superoide anion_induced cytotoxicity. |
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| Keywords: | hSOD gene paraquat xanthine/xanthine oxidase superoxide anion |
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