| ubiCA基因的强化表达及对大肠杆菌辅酶Q生物合成的影响 |
| |
| 引用本文： | 叶江,马林,吴海珍,刘欣毅,张惠展. ubiCA基因的强化表达及对大肠杆菌辅酶Q生物合成的影响[J]. 华东理工大学学报(自然科学版), 2006, 32(3): 269-273 |
| |
| 作者姓名： | 叶江 马林 吴海珍 刘欣毅 张惠展 |
| |
| 作者单位： | 华东理工大学生物反应器工程国家重点实验室,上海,200237;华东理工大学生物反应器工程国家重点实验室,上海,200237;华东理工大学生物反应器工程国家重点实验室,上海,200237;华东理工大学生物反应器工程国家重点实验室,上海,200237;华东理工大学生物反应器工程国家重点实验室,上海,200237 |
| |
| 摘 要： | 为调查大肠杆菌高产辅酶Q(CoQ)的可能性,首先研究大肠杆菌生物合成关键基因ubiCA强化表达对大肠杆菌CoQ产量的影响。本文构建了含有ubiCA基因的5个表达质粒,将其分别转入E.coli JM 83或BL 21(DE 3)菌株中,定量分析这些转化子的辅酶Q产量。结果表明ubiCA基因在ubiC自身启动子(pub iCA-lacZF)介导下的表达最有效,重组菌CoQ产量达到对照的3.5倍,由此推测ubiCA基因的表达可能与其启动子序列有关。
|
| 关 键 词： | 大肠杆菌 ubiCA 基因克隆 强化表达 辅酶Q |
| 文章编号： | 1006-3080（2006）03-0269-05 |
| 收稿时间： | 2005-03-18 |
| 修稿时间： | 2005-03-18 |
Effect of Overexpressing ubiCA Genes Responsible for Ubiquinone Biosynthesis on Ubiquinone Production in Escherichia coli |
| |
| YE Jiang,MA Lin,WU Hai-zhen,LIU Xin-yi,ZHANG Hui-zhan. Effect of Overexpressing ubiCA Genes Responsible for Ubiquinone Biosynthesis on Ubiquinone Production in Escherichia coli[J]. Journal of East China University of Science and Technology, 2006, 32(3): 269-273 |
| |
| Authors: | YE Jiang MA Lin WU Hai-zhen LIU Xin-yi ZHANG Hui-zhan |
| |
| Abstract: | To investigate the possibility of high-level production of UQ,ubiCA genes, which encodes enzymes for the first two steps of UQ biosynthesis in Escherichia coli were overexpressed.Five expression plasmids containing ubiCA were constructed and transformed into E.coli JM83 or BL21(DE3).Different expression of ubiCA genes resulted in an increase in UQ production to various degrees.Among them the expression under ubiC promoter(pubiCA-lacZF) was most effective. Accordingly we succeeded in achieving a level of UQ production(3.5) times that of the control.Meanwhile,the result suggests that the high-level expression of ubiCA gene is probably related to the sequence of its own promoter. |
| |
| Keywords: | Escherichia coli ubiCA cloning overexpression ubiquinone |
| 本文献已被 CNKI 维普 万方数据 等数据库收录！ |
|
