高产杆菌肽菌株的筛选及培养条件研究

采用细胞融合技术得到的菌株与不同来源地衣芽孢杆菌菌株,进行深层液体发酵生产杆菌肽,研究了不同菌株,不同碳源,不同量锌和磷量诸因素对杆菌肽高产的影响.结果表明,1号菌比B-1号、B-2号、3号、4号株菌的效价高,为5.80×102Iu.mL-1.以柠檬酸为碳源时,磷的用量为0.10~0.20 mmol.L-1的最佳培养基中培养时,最有利于杆菌肽的合成,锌的用量10%,其发酵液中杆菌肽效价最高,可达8.86×102~9.85×102Iu.mL-1.

High Yield Bacitracin Strains Screeing and Culture Condition

This research has used the different origin Bacillus licheniformis strains,and the in-depth liquid fermentation production bacitracin and has studied the different strains,different carbon sources,different commensurability zinc and phosphorus to bacitracin high production effect.The result indicats that it is 5.80×102 Iu·mL-1,by using 1# Bacillus licheniformis fermentation,when taking citric acid as carbon source,the phosphorus amount used is 0.10～0.20 mmol·L-1 and zinc amount is 10%,it is most helpful for the bacitracin synthesis,and its fermentation fluid the bacitracin effect price is the highest,it may reach 8.86×102～9.85×102 IU·mol-1.