武汉地区丙型肝炎病毒包膜蛋白E1的生物信息学研究

使用生物信息学的方法，分析武汉地区不同基因型、亚型的丙肝病毒的包膜E1蛋白，预测其二级结构、核苷酸变异性、糖基化位点、亲水性、跨膜区、信号肽、蛋白修饰位点、B细胞抗原.结果显示各HCV的包膜E1蛋白二级结构差距不大；序列始末段有数个氨基酸残基的差距，序列上存在高变异位点，基因型2a的型内一致性最高；序列大量糖基化，有多个糖基化基化位点；序列分布着亲水性区域，基因型1b的分值很高；基因型1b、6a、3b、3a多数亚型有1个跨膜区，基因型2a多数亚型有两个跨膜区；基因型1b、6a、3b、3a无信号肽，基因型2a都有信号肽；蛋白序列上存在多个不同修饰位点和B细胞抗原，各基因型、亚型之间有明显差距，具有较大异质性.该研究为揭示病毒感染机制和研制地区性疫苗提供一定的科学依据.

Bioinformatic study of hepatitis C virus envelope protein E1 in Wuhan

The envelope proteins E1 of different HCV genotypes and subtypes in Wuhan were analyzed bioinformatically to predict their secondary structure， nucleotide variability， glycosylation site， hydrophilicity， transmembrane domain， signal peptide， protein modification site， and B cell antigen. The results indicated that envelope proteins E1 of HCVs possess little secondary structure difference； the beginning segment of the sequence is different from the end segment by several amino acid residues， with hypervariable site in the sequence； the genotype 2a shows the highest consistency； the sequence shows significant glycosylation with multiple glycosylation sites； hydrophilicity areas distribute in the sequence and the genotype 1b shows high score； most subtypes of genotypes 1b， 6a， 3b， 3a have one transmembrane domain， while 2a have two； genotypes 1b， 6a， 3b， 3a have no signal peptide， while 2a has； multiple different modification sites and B cell antigens in the protein sequence， which varies with the genotypes and subtypes， exhibiting significant heterogeneity. The study has provided references for demonstrating mechanism of HCV infection and development of region-specific vaccines.