宁夏干红葡萄酒中反式白藜芦醇的含量测定

以宁夏三大品牌干红葡萄酒为研究对象，采用高效液相色谱法对其中的反式白藜芦醇含量进行了测定，其中，对提取条件和色谱条件进行了优化.结果表明：该方法的最低检出限为0.015 mg/L，定量限为0.03 mg/L；在0.03 mg/L～300 mg/L浓度范围内线性良好，线性方程为y＝1.1161x＋0.0137，R2＝0.9996；重复性较好，标品的RSD为0.23%，样品的RSD为0.18%；准确度较高，加标回收率范围在92.3%～97.1%之间；研究表明反式白藜芦醇的含量与酒的品牌、葡萄品种有较大的关系，供试样品中反式白藜芦醇含量在1.97 mg/L～3.70 mg/L之间.

Determination of trans-resveratrol in Ningxia dry red wine

Taking three brands of Ningxia dry red wine as the research object， the content of trans-resveratrol was determined by HPLC with the extraction conditions and chromatographic conditions optimized. The results showed that the detection limit of this method is 0.015 mg/L and the quantitative limit is 0.03 mg/L. Good linearity in the range of 0.03 mg/L－300 mg/L， with the linear equation of y＝1.1161x＋0.0137 and correlation coefficient of 0.999 6. The reproducibility is good， and the RSD of the standard product is 0.23% and the RSD of the sample is 0.18%. The accuracy is high， and the recovery range is between 92.3%－97.1%. The results showed that the content of trans-resveratrol was closely related to the wine brand and grape variety， and the content of trans-resveratrol in the sample was between 1.97 mg/L－3.70 mg/L.