An anther-specific chalcone synthase-like geneD5 related to rice pollen development |
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Authors: | Honghong Zheng Lijia Qu Meihua Liu Yi Zhang Yunping Shen Junming Wei Naisui Pan Hongya Gu Zhangliang Chen |
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Institution: | (1) The National Laboratory of Protein Engineering and Plant Genetic Engineering, Peking University, 100871 Beijing, China |
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Abstract: | It was shown in a previous analysis that D5 gene from rice (Oryza sativa L.) was an anther-specific gene encoding a chalcone synthase-related protein. In this study, D5 gene was found specifically expressed in tapetum cells as well as in the peripheral cells of the vascular bundle of rice anthers by RNA in situ hybridization. In order to study its function, D5 was transformed into rice in both sense and antisense directions driven by a rice Actin 1 promoter. It has been observed that the pollen grains from the antisense D5 transgenic rice plants are abnormal, indicating that D5 plays a critical role in rice pollen development. |
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Keywords: | antisense in situ hybridization pollen development Oryza sativa |
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