A practicable variant of the ion exchange method for the radiometric estimation of ornithine decarboxylase activity |
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| Authors: | L. W. D. Weber |
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| Affiliation: | (1) Institut für Biologie, Abteilung für Nuklearbiologie, Gesellschaft für Strahlen- und Umweltsforschung m.b.H. München, D-8042 Oberschleissheim, Federal Republic of Germany;(2) Present address: Department of Pharmacology, Toxicology and Therapeutics, University of Kansas Medical Center, 39th and Rainbow Blvd, 66 103 Kansas City, Kansas, USA |
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| Abstract: | Summary A known ornithine decarboxylase assay working with ion exchang separation of [3H]ornithine and [3H]putrescine has been revised. The assay can be performed in disposable 1.5 ml vessels with a total of four pipetting steps. The separation of enzyme substrate and product, respectively, requires 3 h per 50 samples. The detection limit is about 50 pmoles [3H]putrescine formed. |
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| Keywords: | Ornithine decarboxylase radiometric enzyme assay ion exchange separation Michaelis constants pyridoxal phosphate |
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