三硝基丙酸诱导大鼠亨廷顿氏病模型的改进研究

目的评价两种不同的三硝基丙酸(3-Nitropropionic Acid,3-NP)给药方式所致的亨廷顿氏病(Huntington’sdisease,HD)亚急性动物模型的作用。方法通过对24只SD大鼠连续给药和间断给药方式进行比较,连续给药组3-NP 15 mg/kg/d连续5 d腹腔注射;间断给药组给予3-NP 15 mg/kg/d腹腔连续注射2 d后,间隔2 d,再连续注射3 d。结果改良的间断给药方式可明显降低动物死亡率(25%);并且通过Fluro-Jade B染色,可迅速特异地检测到纹状体变性的神经元。结论三硝基丙酸间断给药方式可有效建立HD动物模型。

Study of the Rat Model of Huntington's Disease Induced by 3-Nitropropionic Acid

Objective To evaluate the efficiency of two different administrations of 3-Nitropropionic acid(3-NPA) for inducing rat model of Huntington's disease.Methods Twelve SD rats were divided into two groups,one receiving continuous administration of 3-NPA(15 mg/kg daily,ip,for 5 days) and the other receiving intermittent administration(15 mg/kg daily,ip,for 2 days,no treatment for 2 days,and then 15 mg/kg daily,ip,for 3 days).Results Mortality rate was decreased to 25% and the degenerating cell bodies and affected fibers in striatum were found rapidly by Fluro-Jade B staining in the intermittent administration group.Conclusion The intermittent administration of 3-NPA is an effective method for inducing animal model of Huntington's disease.