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Atomic force microscopic observation on substructure of pollen exine in Cedrus deodara and Metasequoia glyptostroboides
Authors:XING Shuping  LI Bingshi  WANG Chen  HU Yuxi  LIN Jinxing
Abstract:The substructure of pollen exine in Cedrus deodara (Roxb.) Loud. and Metasequoia glyptostroboides Hu et Cheng has been examined with an atomic force microscope (AFM). The results indicate that the exine substructure units containing sporopollenin in two species are similar in shape, which are granular, but slightly different in size. In Cedrus the substructure unit of pollen exine appears to be 56-99 nm long and 42-74 nm wide, while in Metasequoia it appears to be 81-118 nm long and 43-98 nm wide. It has been observed that the subunits of pollen exine in Cedrus arranged tightly to form short-rod-like or spheroidal pollen exine units, several or more than ten of which formed an island-like structure. There are various spaces among these island-like structures which are interconnected to occupy the entire pollen exine. In Metasequoia, the subunits of pollen exine also arranged tightly with a distribution tendency of cluster of 3-10, however, no obvious boundary exists among these clusters. From our results, it is concluded that there is no tendency of helical arrangement for the subunits of pollen exine in Cedrus and Metasequoia, and the results support Southworth' view that subunits of pollen exine are granular shape in lattice structure.
Keywords:Cedrus deodara  Metasequoia glyptostroboides  atomic force microscope  pollen exine substructure  sporopollenin
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