滴水珠组培快繁的实验研究

目的:通过滴水珠组织培养及快速繁殖,解决滴水珠的资源匮乏问题。方法:以滴水珠的珠芽和叶片及叶柄为外植体,进行愈伤组织诱导、愈伤组织分化、丛生芽的增殖、生根诱导及组培苗的移栽研究。采用酸性染料比色法测定滴水珠愈伤组织中总生物碱的含量。结果:滴水珠组织培养的最佳外植体为叶片,在MS+6-BA 2.0 mg/L+NAA 0.2 mg/L的培养基上可以较好地诱导出滴水珠的愈伤组织,滴水珠愈伤分化及丛生芽增殖的最适培养基为MS+6-BA 0.5mg/L+NAA 0.25 mg/L,生根诱导最适培养基为1/2MS+NAA 0.25 mg/L。在培养基中添加100g/L的香蕉汁具有促进滴水珠丛芽增殖及生根的作用。滴水珠愈伤组织中的总生物碱含量为0.784 8%。结论:初步建立了完整的滴水珠的组织培养体系,并成功移栽。

A Study on Rapid Propagation Technology of Cordate Pinellia Tuber

Objective:To solve the lack of resoures of Cordate Pinellia Tuber through tissue culture and rapid propagation technology.Method: Using the bulbule,leaves and leafstalk of Cordate Pinellia Tuber as explants to study the technology of callus induction,callus differentiation,Clustered buds proliferation,root induction and transplantation of tissue culture seedlings;to determine the total alkaloid content in Cordate Pinellia Tuber callus using Acid dye colorimetry.Results: Leaves were the best tissue culture explants of Cordate Pinellia Tuber,MS+6-BA 2.0 mg/L+NAA 0.2 mg/L is a good medium to induce callus of Cordate Pinellia Tuber.The best callus differentiation and Clustered buds proliferation medium is MS+6-BA 0.5 mg/L+NAA 0.25 mg/L,and the best roots induce medium is 1/2MS+NAA 0.25 mg/L.It can promote the Clustered buds proliferation and root grows when added 100 g/L to the medium.The total alkaloid in Cordate Pinellia Tuber callus is 0.784 8%.Conclusion: a rapid propagation technology of Cordate Pinellia Tuber is established in this study,and the transplantation was successful.