| 绿色木霉LTR-2的ATMT插入突变及5,6-二氢-6-戊基-2H-吡喃-2-酮高产菌株的筛选 |
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| 引用本文： | 扈进冬,周红姿,张彦杰,杨合同,陈凯,李纪顺.绿色木霉LTR-2的ATMT插入突变及5,6-二氢-6-戊基-2H-吡喃-2-酮高产菌株的筛选[J].山东科学,2010,23(6):39-43. |
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| 作者姓名： | 扈进冬 周红姿 张彦杰 杨合同 陈凯 李纪顺 |
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| 作者单位： | 1.山东省科学院中日友好生物技术研究中心，山东 济南 250014；
2.山东理工大学生命科学学院，山东 淄博 255049；
3.山东省科学院生物研究所，山东 济南 250014 |
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| 基金项目： | 国家“863”计划现代农业技术领域重大项目资助(2006AA10A211) |
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| 摘 要： | 通过土壤杆菌介导的T-DNA转化方法,利用土壤杆菌菌株携带的Ti质粒,对绿色木霉LTR-2进行插入突变,获得木霉LTR-2突变体共400株。以串珠镰孢菌为指示菌,采用抑菌圈方法,从中筛选吡喃酮高产突变体6株,PCR和探针杂交证实,T-DNA序列已经插入到木霉LTR-2基因组。经GC-MS分析发现,其中一株突变体T-54在PDA培养基上产生的吡喃酮含量较高,在分生孢子中的含量达到了2.62 mg/g,比出发菌株提高9倍。
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| 关 键 词： | 插入突变 吡喃酮 气相色谱-质谱 |
| 收稿时间： | 2010-06-22 |
Insertional Mutation of Trichoderma viride LTR-2 by ATMT and Screening of High-Yield Strains of 5,6-dihydro-6-pentyl-2H-pyran-2-one |
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| HU Jin-dong,ZHOU Hong-zi,ZHANG Yan-jie,YANG He-tong,CHEN Kai,LI Ji-shun.Insertional Mutation of Trichoderma viride LTR-2 by ATMT and Screening of High-Yield Strains of 5,6-dihydro-6-pentyl-2H-pyran-2-one[J].Shandong Science,2010,23(6):39-43. |
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| Authors: | HU Jin-dong ZHOU Hong-zi ZHANG Yan-jie YANG He-tong CHEN Kai LI Ji-shun |
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| Institution: | 1.Biotechnology Center of Shandong Academy of Sciences, Jinan 250014, China;
2.School of Life Sciences, Shandong University of Techology, Zibo 255049, China;
3.Biology Institute of Shandong Academy of Sciences, Jinan 250014, China |
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| Abstract: | We performed insertional mutation for Trichoderma viride LTR-2 by Agrobacteria tuefaxiens-mediated transformation and obtained 400 mutants.We selected six high-yield mutants from these four hundred mutants by powder bacteriostatic circle method.These mutants were identified and analyzed by gas chromatography and mass spectrometric detection.The content of 5,6-dihydro-6-pentyl-2H-pyran-2-one is 2.62 mg/g,which is produced by one mutant T-54 in a PDA plate and is nine times as that by LTR-2. |
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| Keywords: | insertional mutation 5 6-dihydro-6-pentyl-2H-pyran-2-one GC/MS |
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