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Evidence for the presence of viable endothelial cells in cultures derived from dissociated rat brain
Authors:P Panula  F Joó  L Rechardt
Institution:(1) Department of Anatomy, University of Helsinki, Siltavuorenpenger 20 b A, SF-00170 Helsinki 17, (Finland);(2) Present address: Institute of Biophysics, Biological Research Center, Szeged, Hungary
Abstract:Summary The morphology and histochemistry of dissociated newborn rat brain was studied in tissue culture. Direct microscopy of developing cells, electron microscopy and the alkaline phosphatase activity were used to identify the capillary endothelial cells.Acknowledgments. We thank Dr J. Gripenberg for technical assistance. This research was supported by Finnish Cultural Foundation and carried out during the tenure of a fellowship provided for F. J. from the Finnish-Hungarian Cultural Exchange Program.
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