| 河南花背蟾蜍核型、Ag-NORs与性别关系 |
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| 引用本文： | 董自梅,孙小玲,郭培军.河南花背蟾蜍核型、Ag-NORs与性别关系[J].河南师范大学学报(自然科学版),2009,37(1). |
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| 作者姓名： | 董自梅 孙小玲 郭培军 |
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| 作者单位： | 濮阳职业技术学院,河南,濮阳,457000 |
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| 基金项目： | 河南省高等学校青年骨干教师资助计划 |
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| 摘 要： | 利用骨髓细胞蒸汽固定法制备染色体标本,研究了分布于河南新乡的花背蟾蜍(Bufo raddei)的核型,Ag-NORs.结果表明:河南产花背蟾蜍体细胞染色体数为22条,核型公式为2n=22=20m+2sm,全部为中部或亚中部着丝点染色体,NF=44.Ag-NORs结果显示在性成熟的雌雄个体之间出现了稳定差异,提示河南花背蟾蜍可能为同形异配ZW型性别决定.
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| 关 键 词： | 花背蟾蜍 核型 Ag-NORs 性别决定 |
Karyotype,Ag-NORs and Sex Determination of Bufo raddei from Henan |
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| DONG Zi-mei,SUN Xiao-ling,GUO Pei-jun.Karyotype,Ag-NORs and Sex Determination of Bufo raddei from Henan[J].Journal of Henan Normal University(Natural Science),2009,37(1). |
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| Authors: | DONG Zi-mei SUN Xiao-ling GUO Pei-jun |
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| Institution: | Puyang Vacation-technical College;Puyang 457000;China |
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| Abstract: | The Chromosome is prepared from metaphase phase of marrow cells by the steam-fixation method.Karyotype and Ag-NORs are studied on Bufo raddei which distributed in Xinxiang Henan.The diploid number of Bufo raddei is 2 n=22=20 m+2sm,NF=44 and all of the chromosomes are metacentric or submetacentric.Stable diversity of female and male is found in Ag-NORs,showing the sex determination about Bufo raddei is ZW-type. |
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| Keywords: | Ag-NORs |
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