| 肿瘤相关基因对大肠肿瘤临床诊断及预后应用的研究 |
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| 引用本文： | 王中易 赵金伟. 肿瘤相关基因对大肠肿瘤临床诊断及预后应用的研究[J]. 北华大学学报(自然科学版), 2000, 1(2): 122-124 |
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| 作者姓名： | 王中易 赵金伟 |
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| 作者单位： | [1]吉林省人民医院 [2]吉林省武警总队医院 |
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| 基金项目： | 吉林省科委资助项目!(970 334 ) |
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| 摘 要： | 目的 探讨Ｒａｓ，Ｐ５３基因对大肠肿瘤的早期诊断及预后的临床应用价值。方法 用聚合酶链反应－单链构象多态性（ＰＣＲ－ＳＳＣＰ）ＥＢ染色技术对２５例大肠肿瘤原发灶组织进行了Ｋｉ－，Ｎ－ｒａｓ第１处显子和Ｐ５３基因５～８外显子的点突变进行检测。息肉性腺瘤组织中１／２（５０％）发生了Ｋｉ－ｒａｓ点突变。在大肠癌组织中，存在Ｎ－ｒａｓ点突变、Ｋｉ－ｒａｓ点突变、Ｐ５３基因５～８外显子突变，大肠肿瘤组织存在
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| 关 键 词： | 大肠肿瘤 Ras基因 肿瘤相关基因 诊断 预后 |
Tumor Related Genes on Clinical Diagnosis and Prognosis Application of Large Intestine Tumors |
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| Wang Zhongyi ,Zhao Jinwei ,Wang Xinping ,Chen Yuqin ,Sun Ming. Tumor Related Genes on Clinical Diagnosis and Prognosis Application of Large Intestine Tumors[J]. Journal of Beihua University(Natural Science), 2000, 1(2): 122-124 |
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| Authors: | Wang Zhongyi Zhao Jinwei Wang Xinping Chen Yuqin Sun Ming |
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| Affiliation: | Wang Zhongyi 1,Zhao Jinwei 2,Wang Xinping 1,Chen Yuqin 3,Sun Ming 4 |
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| Abstract: | Objective To investigate Ras,P53 genes on clinical diagnosis and prognosis of large intestine tumors.Methods Using a polymerase chain reaction simple strand conformation polymorphism (PCR SSCP) EB staining technique,25 human coloretal tumors for mutation in exons 5,6,7,8 of P53 genes and exon 1 of Ki ,N ras were checked.Results Two polypoid adenoma cases were found Ki ras point mutation positive.In coloretal cancer teste,there were N ras mutation,Ki ras point mutation,and P53 genes mutation positive.Conclusion Ki ras point mutation existed in each stage early diagnosis in coloretal tumors.P53 exons point mutation rate was associated with metastasis of large intestine tumor. |
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| Keywords: | Large intestine tumor Ras gene P53 gene PCR SSCP |
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