| 离子交换色谱流动相组成对溶菌酶复性的影响 |
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| 引用本文： | 王彦,耿信笃.离子交换色谱流动相组成对溶菌酶复性的影响[J].西北大学学报,2004,34(1):47-51. |
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| 作者姓名： | 王彦 耿信笃 |
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| 作者单位： | 西北大学现代分离科学研究所／现代分离科学陕西省重点实验室，陕西西安710069 |
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| 基金项目： | 国家自然科学基金资助项目(20175016) |
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| 摘 要： | 目的 研究了复性缓冲液组成对还原变性溶茵酶复性的影响。方法用离子交换色谱法对还原变性溶菌酶进行复性。结果低离子强度的Tris缓冲液比高离子强度的PBS缓冲液能明显提高复性收率；当复性缓冲液中不合其他种类的盐时，脲浓度为2．0mol／L时复性产率最高；当脲浓度低时，复性缓冲液中含有氯化钠和硫酸铵的复性效果均不如不合这些盐的复性效果；当脲浓度高时，硫酸铵能很好地提高溶菌酶的复性回收率。结论认为Hofmeister效应是造成这些现象的主要原因。
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| 关 键 词： | 离子交换色谱 复性缓冲液 溶菌酶 脲 盐 |
| 文章编号： | 1000-274X(2004)01-0047-05 |
| 修稿时间： | 2002年12月10 |
Effects of the composition of mobile phase on the bioactivity recovery of reduced/denatured of lysozyme by using ion-exchange chromatography |
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| Abstract: | Aim The effects of the composition of mobile phase on the bioactivity recovery of reduced/denatured of lysozyme was studied.MethodsThe refolding of the reduced denatured lysozyme by ion exchange chromatography.ResultsIt was found that the bioactivity yield in the Tris buffer with low ion strengthen is better than that of in the phosphate solution buffer (PBS) with high ion strengthen.When the renaturation buffer includes any other kinds of salt, the bioactivity recovery is the highest with 2.0 mol/L urea concentration in the renaturation buffer.The bioactivity recovery of lysozyme is bad when the renaturation buffer contains other kinds of salt except urea.However,ammonium sulfate can enhance the bioactivity yield of reduced/denatured lysozyme when urea concentration is higher in the renaturation buffer.ConclusionThe reason of these phenomena is discussed in detail.Hofmeister effect is the main reason. |
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