| 猪hnRNPK基因启动子的克隆及序列分析 |
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| 引用本文： | 马超锋,梁小娟,王明玉,等. 猪hnRNPK基因启动子的克隆及序列分析[J]. 信阳师范学院学报(自然科学版), 2014, 0(1): 27-30 |
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| 作者姓名： | 马超锋 梁小娟 王明玉 等 |
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| 作者单位： | ;1.信阳市动物疫病预防控制中心;2.信阳师范学院生命科学学院 |
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| 摘 要： | 首次克隆了猪hnRNPK基因启动子序列,并进一步对该序列进行了分析.结果显示:该基因启动子约1 kb,与已报道的人的相应序列相似度为78.9%,具有相同的TCTCGCGAGA核心启动子序列和转录起始位点.利用在线软件分析发现,猪hnRNPK基因启动子不含TATA盒,而含有CAAT盒的GC富集区,存在两处CpG岛,具有SP1、UCE.2、GCF、EARLY-SEQ1、TTR_inverted_repeat、NGFI-C、EARLY-SEQ1等多种转录因子潜在结合位点,并且具有8种基序结构.
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| 关 键 词： | 猪 hnRNPK 启动子 转录因子 |
Cloning and Sequence Analysis of the Promoter Region of Porcine hnRNPK Gene |
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| Affiliation: | ,Xinyang Animal Disease Control and Prevention Center,College of Life Sciences,Xinyang Normal University |
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| Abstract: | The porcine hnRNPK gene promoter were cloned firstly and analysis by the bioinformatics software. The results showed that the length of porcine hnRNPK promoter is about 1 kb,and had 78. 9 percent of the similarity comparing with human hnRNPK promoter,which had the same core promoters' sequence of TCTCGCGAGAand transcriptional start site. The results of bioinformatics analysis showed that no TATA box and two CpG island are found in porcine hnRNPK gene promoter region,and exited many transcription factors-binding sites such as SP1,UCE. 2、GCF,EARLY-SEQ1,TTR_inverted_repeat,NGFI-C,and 8 motifs. |
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| Keywords: | pig hnRNPK promoter transcription factor |
| 本文献已被 CNKI 等数据库收录！ |
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