| 米根霉发酵产L-乳酸体系中淀粉酶的研究 |
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| 引用本文： | 潘丽军,张志英,姜绍通,潘利华. 米根霉发酵产L-乳酸体系中淀粉酶的研究[J]. 合肥工业大学学报(自然科学版), 2006, 29(1): 113-116 |
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| 作者姓名： | 潘丽军 张志英 姜绍通 潘利华 |
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| 作者单位： | 合肥工业大学生物与食品工程学院,安徽,合肥,230009;合肥工业大学生物与食品工程学院,安徽,合肥,230009;合肥工业大学生物与食品工程学院,安徽,合肥,230009;合肥工业大学生物与食品工程学院,安徽,合肥,230009 |
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| 基金项目： | 安徽省重点科技攻关资助项目 , 安徽省自然科学基金 |
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| 摘 要： | 米根霉As3.819是一株L-乳酸的高产菌株,且能够分泌淀粉酶直接利用淀粉发酵生产乳酸。文章研究了不同培养基主要成分下米根霉菌株As3.819生产L-乳酸和淀粉酶情况,并对发酵液中淀粉酶水解产物进行分析。研究结果表明,在甘薯淀粉8%、硫酸铵为0.4%时L-乳酸和淀粉酶的产量相对较高,且在培养基中加入麸皮后能增加淀粉酶的分泌,种子培养基中碳源为麦芽糖时也能起到很好的效果。
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| 关 键 词： | 米根霉 L-乳酸 淀粉酶 发酵液 |
| 文章编号： | 1003-5060(2006)01-0113-04 |
| 修稿时间： | 2005-03-22 |
Study of amylase in L-lactic acid fermentation with Rhizopus oryzae |
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| PAN Li-jun,ZHANG Zhi-ying,JIANG Shao-tong,PAN Li-hua. Study of amylase in L-lactic acid fermentation with Rhizopus oryzae[J]. Journal of Hefei University of Technology(Natural Science), 2006, 29(1): 113-116 |
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| Authors: | PAN Li-jun ZHANG Zhi-ying JIANG Shao-tong PAN Li-hua |
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| Abstract: | In order to obtain the best L-lactic acid product of Rhizopus oryzae,the growth of Rhizopus oryzae As3.819 in different culture mediums under different culture conditions and the production of amylase were studied.The product hydrolyzed by amylase in broth was also analyzed.The results of this experiment indicate that the outputs of L-lactic acid and amylase were highest when using 8% of sweet potato starch as the carbon substrate and 0.4% of(NH_4)_2SO_4 as the nitrogen substrate.The addition of bran and using maltose as the carbon substrate could induce the secretion of amylase. |
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| Keywords: | Rhizopus oryzae L-lactic acid amylase culture medium |
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