| Pichia pastoris表达系统提高表达量的几个关键点 |
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| 引用本文： | 周细南,张爱联,张添元,罗进贤,谭燕华. Pichia pastoris表达系统提高表达量的几个关键点[J]. 海南大学学报(自然科学版), 2008, 26(1): 68-73 |
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| 作者姓名： | 周细南 张爱联 张添元 罗进贤 谭燕华 |
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| 作者单位： | 中国热带农业科学院,热带生物技术研究所,海南,海口,571101;海南大学,农学院,海南,海口,570228;中国热带农业科学院,热带生物技术研究所,海南,海口,571101;中山大学,生命科学学院,广东,广州,510275 |
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| 摘 要： | Pichia pastoris是近年来迅速发展的一种真核生物表达宿主，有着广阔的应用前景．本文从提高Ppastoris表达重组蛋白产量的角度，就启动子、高拷贝重组子、密码子偏爱、宿主菌和高密度发酵等方面的优化进行了综述．
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| 关 键 词： | Pichia pastoris 表达系统 产量 |
| 文章编号： | 1004-1729(2008)01-0068-06 |
| 修稿时间： | 2007-09-09 |
Some Key Points of Improvement of Pichia pastoris Expression System |
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| ZHON Xi-nan,ZHANG Ai-lian,ZHANG Tian-yuan,LUO Jin-xian,TAN Yan-hua. Some Key Points of Improvement of Pichia pastoris Expression System[J]. Natural Science Journal of Hainan University, 2008, 26(1): 68-73 |
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| Authors: | ZHON Xi-nan ZHANG Ai-lian ZHANG Tian-yuan LUO Jin-xian TAN Yan-hua |
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| Affiliation: | ZHON Xi-nan, ZHANG Ai-lian, ZHANG Tian-yuan, LUO Jin-xian,TAN Yan-hua ( 1. Institute of Tropical Bioscience and Biotechnology , National Key Biotechnology Laboratory for Tropical Crops, CATAS, Haikou 571101, China ; 2. College of Life Science and Agriculture, Hainan University, Haikou 570228, China; 3. The Key Laboratory of Gene Engineering of Ministry of Education and Department of Biochemistry, Sun Yat-Sen University , Guangzhou 510275, China) |
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| Abstract: | Pichia pastoris has been proved to be a promising host organism for the production of heterogonous proteins. We report here the optimization of promoter, high copy integrants, codon preference, host genotype, and high-density cell culture of Pichia pastoris to increase the production of hetcrogonous proteins and make it more efficient for large-scale production. |
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| Keywords: | Pichia pastoris Expression system Output |
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