分子生物学方法在微生物生态学研究中的应用

由于传统分离培养微生物的局限，分子生物学技术避开了传统微生物培养分析的环节，直接从样品中抽取总DNA，然后通过PCR扩增及其相关技术、核酸杂交技术、RNA基因序列分析等方法对直接提取的总DNA进行分析，了解其中微生物信息．这些通过遗传物质进行研究的分子方法，为微生态的研究开辟了新的途径，并已经得到广泛的应用．

Molecular Biology Method in Microorganism Ecology Research Application

Because of the difficulty associated with traditional isolating and culturing bacteria,but the molecular techniques based on culture-independent techniques,extract directly the variable genomic DNA from microbial samples and then analysis the genetic information,by the amplification of polymeric chain reaction(PCR) nucleic acid hybridizerRNA sequence and other methods.At present,these molecular techniques have opened up the new way and been widely used in the research of microbial ecology.