长期精神紧张建立抑郁症模型

目的 采用长期精神紧张刺激建立动物抑郁症模型。方法 选用C57BL/6 J雄性4周龄小鼠17只,体质量(11. 05±1. 65) g,随机分为对照组、长期精神紧张刺激(LTMS)组。LTMS组单笼饲养并连续21 d每天给予猫惊吓3 h和剥夺睡眠12 h。实验组通过测定摄食量变化和高架十字迷宫实验、旷场实验、强迫游泳行为学变化来判断建模是否成功。结果 与对照组相比,LTMS组摄食量明显降低(P<0. 01),强迫游泳不动时间显著延长(P<0. 01)。旷场试验中,LTMS组小鼠穿越格数和直立次数均明显少于对照组,差异具有极显著性(P<0. 01)。高架十字迷宫实验中,LTMS组小鼠闭合臂停留时间明显延长,差异具有极显著性(P<0. 01);进入开臂次数比例减少,差异具有显著性(P<0. 05)。结论 长期精神紧张模型表现出行为绝望、活动能力下降、兴趣丧失、焦虑与抑郁症临床表现相似的症状,可作为抑郁症模型。

Model Depression for Chronic Mental Stress

Objective Depression model was established with long-term mental stress stimulation. Methods 14 male C57BL/6J mice aged 4 weeks (body weight 11.05 1.65 g) were randomly divided into control group and LTMS group. In the LTMS group, the cats were kept in a single cage for 21 consecutive days and were given shock for 3h and sleep deprivation for 24h.The experimental group judged whether the modeling was successful or not by measuring the change of food intake, the overhead cross maze experiment, the open field experiment and the change of forced swimming behavior. Results Compared with the control group, the food intake of LTMS group was significantly reduced (p<0.01), and the forced swimming time was significantly prolonged (p<0.01).In the open-field experiment, the number of traversal and the number of upright position of LTMS group mice were significantly less than that of the control group, and the difference was extremely significant (p<0.01).In the overhead cross maze experiment, the residence time of the closed arm of LTMS group mice was significantly shortened, and the difference was extremely significant (p<0.01), and the proportion of the times of entering the open arm was reduced, and the difference was significant (p<0.05). Conclusions The long-term mental stress model can be used as the depression model because it shows the similar symptoms of behavior despair, decreased activity ability, loss of interest, anxiety and clinical manifestations of depression.