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不同碳源对内生真菌Shiraia sp. Slf14产苝醌类色素的影响
引用本文:汤丽娟1,包建莹1,颜日明1,汪 涯2,杨慧林1,张志斌1,朱 笃1,2. 不同碳源对内生真菌Shiraia sp. Slf14产苝醌类色素的影响[J]. 江西师范大学学报(自然科学版), 2019, 0(5): 490-495. DOI: 10.16357/j.cnki.issn1000-5862.2019.05.09
作者姓名:汤丽娟1  包建莹1  颜日明1  汪 涯2  杨慧林1  张志斌1  朱 笃1  2
作者单位:1.江西师范大学生命科学学院,江西省亚热带植物资源保护与利用重点实验室,江西 南昌 330022; 2.江西科技师范大学生命科学学院,江西省生物加工过程重点实验室,江西 南昌 330013
摘    要:苝醌类化合物是一类具有抗癌、抗病毒活性的光敏性色素.该文比较了不同碳源对蛇足石杉内生真菌Shiraia sp. Slf14液态发酵产苝醌类化合物的影响,结果表明:可溶性淀粉有利于Shiraia sp. Slf14菌体的生长,而果糖则有利于苝醌类化合物的生物合成.当果糖浓度为60 g·L-1时,菌体苝醌类化合物总产量可达1 566.64 mg·L-1,同时果糖能提升竹红菌甲素组分的含量.菊粉作为一种来源于菊芋的果糖基生物质,同样有利于苝醌类色素的生物合成,当其浓度为40 g·L-1时,色素总产量可达1 322.85 mg·L-1,且其有利于痂囊腔菌素A组分积累.

关 键 词:竹黄菌  碳源  苝醌类化合物  竹红菌素  代谢调控

The Effects of Different Carbon Sources on Production of Perylenequinones in Endophytic Fungus Shiraia sp. Slf14
TANG Lijuan1,BAO Jianying1,YAN Riming1,WANG Ya2,YANG Huilin1,ZHANG Zhibin1,ZHU Du1,' target="_blank" rel="external">2. The Effects of Different Carbon Sources on Production of Perylenequinones in Endophytic Fungus Shiraia sp. Slf14[J]. Journal of Jiangxi Normal University (Natural Sciences Edition), 2019, 0(5): 490-495. DOI: 10.16357/j.cnki.issn1000-5862.2019.05.09
Authors:TANG Lijuan1,BAO Jianying1,YAN Riming1,WANG Ya2,YANG Huilin1,ZHANG Zhibin1,ZHU Du1,' target="  _blank"   rel="  external"  >2
Affiliation:1.Key Laboratory of Protection and Utilization of Subtropic Plant Resources of Jiangxi Province,College of Life Sciences,Jiangxi Normal University,Nanchang Jiangxi 330022,China; 2.Key Laboratory of Bioprocess of Jiangxi Province,College of Life Sciences,Jiangxi Science and Technology Normal University,Nanchang Jiangxi 330013,China
Abstract:Perylenequinones(PQ)are a class of photosensitive pigments with antitumor and antiviral activity.In present study,the effects of different carbon sources on the growth and PQ production of the endophytic fungus Shiraia sp.Slf14 are studied.The results show that soluble starch is suitable to fungal growth,while fructose can enhance PQ production significantly.With 60 g·L-1 fructose as the carbon source,the total PQ yield of 1 566.64 mg·L-1 is achieved.Moreover,fructose is also conducive to the accumulation of hypocrellin A(HA)component.Besides,the inulin as a kind of fructose based biomass from Jerusalem artichoke also could improve PQ production.By using 40 g·L-1 inulin,the total PQ yield of 1 322.85 mg·L-1 is obtained,and inulin could enhance the accumulation of elsinochrome A(EA)component.The results of this study will lay a basis for improving PQ fermentation production of Shiraia sp.Slf14.
Keywords:Shiraia bambusicola  carbon sources  perylenequinones  hypocrellins  metabolic regulation
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