高效毛细管电泳分离模型及其应用

应用色谱分离理论，综合考虑了分子扩散、进样量、能量耗散等因素对高效毛细管电泳分离的影响，建立了高效毛细管电泳分离模型。研究了电压、溶液浓度、进样量、毛细管管径等因素对理论极高度的影响，并与模型计算结果进行了比较。结果表明，所提出的模型能够较好地描绘高效毛细管电泳的分离机理，该模型可作为优化操作条件和进行放大设计的基础。在此基础上，应用高效毛细管电泳技术对生物产品进行了分离，以探讨该技术在生物领域的应用。

Study on separation modelling and application of high performance capillary electrophoresis

The separation mechanism of high performance capillary electrophoresis was studied in light of chromatographic separation principle and by considering the effects of molecular diffusion, injection and power dissipation. The expression obtained for the equivalent height of a theoretical plate was compared with the experimental results, which showed that the calculated results using the model were consistent with the experimental data. The model may provide the fundamentals for optimizing experimental conditions and scale-up. Based on the above studies, some bioproducts were separated and analysed by HPCE equipment to find out its application in biological fields.