| hKv4．3基因中抑制序列功能的研究 |
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| 引用本文： | 李皓.hKv4．3基因中抑制序列功能的研究[J].松辽学刊,2008,29(3):69-71. |
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| 作者姓名： | 李皓 |
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| 作者单位： | 吉林工程技术师范学院,吉林长春130052 |
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| 摘 要： | 目的：研究基因中抑制序列对离子通道基因表达的影响．方法：我们将hKv4．3基因的启动子和5’非翻译区的一段序列（＋2-＋160，S160）以不同的位置和方向克隆到报告质粒pβ-gal-Basievector中，瞬时表达，进行β—galactosidase相对活性分析．结果：S160对hKv4．3基因表达具有强烈的抑制作用．结论：抑制序列S160对hKv4．3基因的抑制功能具有位置特异性．
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| 关 键 词： | hKv4.3基因 抑制序列 克隆 |
Study on the Function of Inhibition Sequence in hKv4.3 |
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| LI Hao.Study on the Function of Inhibition Sequence in hKv4.3[J].Songliao Journal (Natural Science Edition),2008,29(3):69-71. |
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| Authors: | LI Hao |
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| Institution: | LI Hao (Jilin Teacher＇ s Institute of Engineering Technology, Changchun 130052, China) |
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| Abstract: | Objective：To study the effect of repressor on ion channel gene expression. Methods：We cloned hKv4.3 promoter and the sequence（ ＋ 2 - ＋ 160, S160）of 5＇UTR of hKv4.3 gene into pβ-gal-Basic vector. Transient expression of the pβ-gal vector and analysis of relative activity of β-galactosidase were undergone. Results： S160 intensively repressed the expression of hKv4.3 gene. Conclusion： We found that the inhibition of S160 to hKv4.3 gene with positionspecificity. |
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| Keywords: | hKv4 3 gene inhibition sequence clone |
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