| 盐源山蛭抗凝血蛋白的分离及抗凝血特性分析 |
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| 引用本文： | 赵荣乐,程新.盐源山蛭抗凝血蛋白的分离及抗凝血特性分析[J].北京师范大学学报(自然科学版),1998,34(1):115-118. |
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| 作者姓名： | 赵荣乐 程新 |
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| 作者单位： | 北京师范大学生物学系 |
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| 摘 要： | 以肝素琼脂糖亲和层析和超速离心分离了盐源山蛭的抗凝血蛋白，抗凝血活性试验证明，该蛋白不抑制凝血因子Ｘａ的活性，而显著抑制凝血酶的活性，亲和层析的蛋白洗脱峰与抗凝血酶的活力峰相吻合，因此，盐源山蛭抗凝血蛋白属于凝血酶特异性的抗凝血蛋白。
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| 关 键 词： | 盐源山蛭 抗凝血蛋白 凝血酶 凝血因子Xa 分离 |
ISOLATION AND CHARACTERIZATION OF AN ANTICOAGULANT PROTEIN FROM YANYUAN LEECH |
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| Zhao Rongle,Cheng Xin,Zhou Yun.ISOLATION AND CHARACTERIZATION OF AN ANTICOAGULANT PROTEIN FROM YANYUAN LEECH[J].Journal of Beijing Normal University(Natural Science),1998,34(1):115-118. |
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| Authors: | Zhao Rongle Cheng Xin Zhou Yun |
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| Abstract: | An anticoagulant protein is isolated from Yanyuan led (Haemendipsa yanyuanensiS) by heparin agarose affinity chromatography and ultracentrifugation. Using the method of chromogenic substrate, the anticoagulant activity of the protein is measured, the results indicate that the inhibitor is thrombin-specific but not factor Xa-specific. The elution peaks at UV 280nm of the protein in the affinity chromatograph are in correspondence with the antithrombin activity peaks at 405nm. So that the anticoagulant protein isolated from yanyuan leech is thrombin-specific anticoagulant protein can be concluded. |
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| Keywords: | Haemendipsa yanyuanensis anticoagulant protein thrombin factor Xa |
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