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转基因鱼的研究及其发展前景
引用本文:杨学明,张立,蒋和生. 转基因鱼的研究及其发展前景[J]. 广西科学, 2006, 13(1): 76-80
作者姓名:杨学明  张立  蒋和生
作者单位:广西水产研究所,广西南宁,530021;广西大学动物科技学院,广西南宁,530005
摘    要:20年来国内外主要进行了快速生长转基因鱼、抗寒(耐寒)转基因鱼、抗病转基因鱼和其他抗性转基因鱼的研究,常用的鱼类基因转移技术有显微注射法、精子载体法、电穿孔法、基因枪法、胚胎干细胞载体、脂质体法和缺陷性病毒感染法等基因转移方法。转基因鱼的安全性和遗传稳定性有望通过进一步的科研攻关得到解决,转基因鱼的生产应用前景广阔。

关 键 词:转基因鱼  显微注射  基因打靶
文章编号:1005-9164(2006)01-0076-05
收稿时间:2005-08-15
修稿时间:2005-08-15

Progress in Transgenic Fish Breeding Research
YANG Xue-ming,ZHANG Li and JIANG He-sheng. Progress in Transgenic Fish Breeding Research[J]. Guangxi Sciences, 2006, 13(1): 76-80
Authors:YANG Xue-ming  ZHANG Li  JIANG He-sheng
Affiliation:1. Guangxi Fisheries Institute, Nanning, Guangxi, 530021, China ; 2. Faculty of Animal Science and Technology, Guangxi University, Nanning, Guangxi, 530005, China
Abstract:Researches in transgenic fish in the past two decades home and abroad are mainly concentrated on several aspects including fast-growth transgenic fish,antifreeze/freeze-resistant transgenic fish and antidisease transgenic fish.Often used technologies for gene transfer in fish are sperm mediated gene transfer,electroporation,particle gun,embryonic stem cell insertion,liposomes,retrovirus infection,and so on.The safey and heredity stability of transgcnic fish could be lifted and finally solved through further research.Transgenic fish have broad and bright prospects in its future application.
Keywords:transgenic fish  microingection  gene targetting
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