| 邻苯三酚自氧化法测定超氧化物歧化酶活性的研究 |
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| 引用本文： | 许雅娟,赵艳景,胡虹. 邻苯三酚自氧化法测定超氧化物歧化酶活性的研究[J]. 西南民族学院学报(自然科学版), 2006, 32(6): 1207-1209 |
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| 作者姓名： | 许雅娟 赵艳景 胡虹 |
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| 作者单位： | 连云港师范高等专科学校自然科学系,江苏,连云港,222006: 江苏省淮海工学院化学工程系,江苏,连云港,222006 江苏省连云港高等中医药技术学校,江苏,连云港,222006 |
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| 摘 要： | 目前超氧化物歧化酶(SOD)活性测定结果混乱,为提高测定结果的可比性,对邻苯三酚自氧化法测定SOD活性的方法进行了研究,就该活力测定体系中缓冲溶液的介质组成、浓度、pH及所含的EDTA量等因素对测定结果的影响进行了探讨,根据实验结果,提出了新的改良方法.
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| 关 键 词： | 酶活力测定 邻苯三酚自氧化 超氧化物歧化酶(SOD) 超氧阴离子自由基 |
| 文章编号： | 1003-2843(2006)06-1207-03 |
| 修稿时间： | 2006-09-07 |
Research on the measurement of the SOD activity via pyrogallol auto-oxidation |
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| XU Ya-juan,ZHAO Yan-jing,HU Hong. Research on the measurement of the SOD activity via pyrogallol auto-oxidation[J]. Journal of Southwest Nationalities College(Natural Science Edition), 2006, 32(6): 1207-1209 |
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| Authors: | XU Ya-juan ZHAO Yan-jing HU Hong |
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| Abstract: | To improve compatibility of the results in assessment of super-oxide dismutase(SOD) activity,the method of pyrograllol auto-oxidation is used to assay SOD activity,and the factors influencing the results of the assessment such as the constituents and concentration of the buffer,pH and EDTA concentration ect.are discussed.According to the investigation on optimum condition,a new assay method of SOD activity is given finally. |
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| Keywords: | activity assay method of enzyme pyrogallol auto-oxidation super-oxide dismutase super-oxide anion radical |
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