| 黄曲霉毒素高污染区肝细胞癌P53基因高频率定点突变 |
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| 引用本文： | 马韵,邓卓霖,罗虹,丁志敏.黄曲霉毒素高污染区肝细胞癌P53基因高频率定点突变[J].广西科学,1996,3(3):48-50. |
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| 作者姓名： | 马韵 邓卓霖 罗虹 丁志敏 |
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| 作者单位： | 广西医科大学病理学教研室 |
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| 摘 要： | 用聚合酶链反应及限制性片段长度多态性（ＰＣＲ＿ＲＦＬＰ）分析技术，对广西黄曲霉毒素高污染２例肝细胞癌（ＨＣＣ）进行分析，检查Ｐ５３基因第７外显子的２４９密码子的突变频率。结果发现３６／５２例ＨＣＣ中２４８密码子有集中的点突变，频率为６９．２％。Ｐ５３基因突变热点与乙型肝炎病毒感染无关。提示黄曲霉毒素Ｂ１是突变热点的主要原因。
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| 关 键 词： | 肝癌 肝细胞癌 P53基因 黄曲霉毒素B1 基因突变 |
| 收稿时间： | 1996/6/10 0:00:00 |
High Frequency of P53 Gene Fixed Point Mutation in Hepatocellular Carcinoma from AFB1 High Risk Area |
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| Ma Yun,Deng Zhuolin,Luo Hong and Ding Zhimin.High Frequency of P53 Gene Fixed Point Mutation in Hepatocellular Carcinoma from AFB1 High Risk Area[J].Guangxi Sciences,1996,3(3):48-50. |
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| Authors: | Ma Yun Deng Zhuolin Luo Hong and Ding Zhimin |
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| Institution: | Dept. of Pathology, Guangxi Medical Univ., 6 Binhu Road, Nanning, Guangxi, 530021,Dept. of Pathology, Guangxi Medical Univ., 6 Binhu Road, Nanning, Guangxi, 530021,Dept. of Pathology, Guangxi Medical Univ., 6 Binhu Road, Nanning, Guangxi, 530021 and Dept. of Pathology, Guangxi Medical Univ., 6 Binhu Road, Nanning, Guangxi, 530021 |
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| Abstract: | Fifty two cases of hepatocellular carcinoma (HCC) from high aflatoxin B1 (AFB1) contaminated area in Guangxi were studied by polymerase chain reaction and restrictive fragment length polymorphism (PCR-RFLP) analysis to examine the exon 7 of P53 gene in HCC. Mutation points were mostly clustered at codon 249 to form a mutational hot-spot. The frequency was 36/52 (69. 2%). It seems that there has no relation to hepatitis B virus infection. The result indicates that AFB1 is the most important agent for the specific mutation at hot-spot in the P53 gene of human hepatocellular carcinoma. |
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| Keywords: | hepatocellular carcinoma P53 gene mutational hot-spot hepatitis B virus aflatoxin B1 |
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