| 重组PAI—2基因在NIH3T3细胞中的活性表达 |
| |
| 引用本文： | 曹祥荣 laug,WE. 重组PAI—2基因在NIH3T3细胞中的活性表达[J]. 南京师大学报(自然科学版), 1994, 17(2): 59-63 |
| |
| 作者姓名： | 曹祥荣 laug WE |
| |
| 作者单位： | 南京师范大学生物系 |
| |
| 基金项目： | 国家自然科学基金，江苏自然科学基金 |
| |
| 摘 要： | 构建了人ＰＡＩ—２ｃＤＮＡ睥达质粒，以ＮＩＨ３Ｔ３为转染宿主细胞，ＮｏｒｔｈｅｒｎＢｌｏｔ，ＷｅｓｔｅｒｎＢｌｏｔ和ＰＡＩ活性扩散试验结果表明人ＰＡＩ—２ｃＤＮＡ可以在ＮＩＨ３Ｔ３细胞中正确表达出ＰＡＩ—２蛋白，且具有抑制ＰＡ的活性，同时未能检测到ＮＩＨ３Ｔ３细胞表达ＰＡＩ—２蛋白质．为探讨ＰＡ—ＰＡＩ—２系统的调控机制打下基础．
|
| 关 键 词： | 基因表达 NIH3T3细胞 PAI－2 |
Expression of Recombinated Human PAI-2 Gene in NIH3T3 Cell |
| |
| Cao Xiangrong, Walter E. Large. Expression of Recombinated Human PAI-2 Gene in NIH3T3 Cell[J]. Journal of Nanjing Normal University(Natural Science Edition), 1994, 17(2): 59-63 |
| |
| Authors: | Cao Xiangrong Walter E. Large |
| |
| Abstract: | In order to study the regulation of PA -PAI - 2 system in the invasion and metastasis of tumor cell, we constructed the human PAI-2 cDNA expression vector which was controlled by MSV-LTR and trans feeted into NIH3T3 cells. The result of Northern Blot, Western Blot and assay of PAl activity state that recombinated PAI-2 cDNA can be expressed in NIH3T3 cells, the expressed product of PAI-2 gene can inhibit PA activity. |
| |
| Keywords: | Gene expression Gene manipulation Plasmin |
| 本文献已被 CNKI 维普 等数据库收录！ |
