| 高效液相色谱法测定吉祥草根茎中芦丁的含量 |
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| 引用本文： | 王倩,邹坤.高效液相色谱法测定吉祥草根茎中芦丁的含量[J].三峡大学学报(自然科学版),2011,33(4):89-91. |
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| 作者姓名： | 王倩 邹坤 |
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| 作者单位： | 天然产物研究与利用湖北省重点实验室三峡大学化学与生命科学学院,湖北宜昌,443002 |
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| 基金项目： | 国家自然科学基金面上项目(30870254,31070313) |
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| 摘 要： | 目的：建立吉祥草根茎中芦丁的含量测定方法.方法：采用高效液相色谱技术测定芦丁的含量,色谱条件为：Diamonsil C18（4.6 mm×250 mm,5μm）色谱柱,乙腈-0.1%磷酸水（28：72）为流动相,恒定流速为1.0 mL/min,检测波长为350 nm,柱温25℃.结果：吉祥草根茎中芦丁的含量为0.26%.芦丁在0.13～0.65 mg/mL范围内与色谱峰面积呈良好的线性关系,r=0.999 9,平均回收率为99.87%,RSD（n=5）为1.41%.结论：该方法简便准确,重现性好,可以作为吉祥草中芦丁的含量测定方法.
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| 关 键 词： | 吉祥草 高效液相色谱法 芦丁 含量测定 |
Content Determination of Rutin in Reineckia Carnea by Using HPLC |
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| Wang Qian,Zou Kun.Content Determination of Rutin in Reineckia Carnea by Using HPLC[J].Journal of China Three Gorges University(Natural Sciences),2011,33(4):89-91. |
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| Authors: | Wang Qian Zou Kun |
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| Institution: | Wang Qian Zou Kun(Hubei Key Laboratory of Natural Products Research & Development,College of Chemistry and Life Sciences,China Three Gorges Univ.,Yichang 443002,China) |
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| Abstract: | The high-performance liquid chromatography(HPLC) is used to determine the rutin content in Reineckia Carnea.The analysis is performed on diamonsil C18(250 mm×4.6 mm.id.,5μm) column in eluation with acetonitrile-0.1% phosphoric acid(28:72) in a flow rate of 1.0 mL/min;and the signals are detected under detecting wavelength of 350 nm and the column temperature is kept at 25 ℃.Contents of rutin in Reineckia Carnea has been determined as 0.26 %.The calibration curve shows a good linear relationship at the range of 0.13-0.65 mg/mL with its linear coefficient of 0.999 9;and the average recovery is determined as 99.87% and RSD(n=5) 1.41%.The method is simple,accurate and repeatable.It can be used for quality control of rutin in Reineckia Carnea. |
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| Keywords: | Reineckia Carnea HPLC rutin content determination |
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